SLC9A1 antibody (C-Term, N-Term)

Catalog No. ABIN500339

The Rabbit Polyclonal anti-SLC9A1 antibody is suitable to detect SLC9A1 in samples from Human, Rat and Mouse. It has been validated for WB, IHC (p) and EIA.

Quick Overview for SLC9A1 antibody (C-Term, N-Term) (ABIN500339)

Target: SLC9A1 (Solute Carrier Family 9, Subfamily A (NHE1, Cation Proton Antiporter 1), Member 1 (SLC9A1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC9A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-SLC9A1 Antibody

Binding Specificity: C-Term, N-Term

Specificity: This antibody detects SLC9A1 / NHE1 at N-term.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Peptide affinity chromatography

Immunogen: Nhe-1 antibody was raised against a 20 amino acid peptide near the carboxy terminus of the human Nhe-1.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for SLC9A1

Target: SLC9A1 (Solute Carrier Family 9, Subfamily A (NHE1, Cation Proton Antiporter 1), Member 1 (SLC9A1))

Alternative Name: SLC9A1 / NHE1

Background: The Na+/H+ antiporter (Nhe-1) is a ubiquitous membrane-bound enzyme involved in pH regulation of vertebrate cells and is specifically inhibited by the diuretic drug amiloride and activated by a variety of signals including growth factors, mitogens, neurotransmitters, and tumor promoters. Nhe-1 acts as an anchor for actin filaments to control the integrity of the cortical cytoskeleton. This occurs through a previously unrecognized structural link between Nhe-1 and the actin-binding proteins ezrin, radixin, and moesin, collectively referred to as ERM proteins. A structural role for Nhe-1 has been proposed in regulating the cortical cytoskeleton that is independent of its function as an ion exchanger. It is also thought that Nhe-1 play a role in hypertension. At least two isoforms of Nhe-1 are known to exist.Synonyms: APNH, APNH1, Na(+)/H(+) antiporter, Na(+)/H(+) exchanger 1, Sodium/hydrogen exchanger 1, Solute carrier family 9 member 1, amiloride-sensitive

Gene ID: 6548

UniProt: P19634

Pathways: Glycosaminoglycan Metabolic Process, Proton Transport