SNTA1 antibody (Intracellular)

Catalog No. ABIN7043797

Product Details anti-SNTA1 Antibody

Target: SNTA1 (Syntrophin, alpha 1 (Dystrophin-Associated Protein A1, 59kDa, Acidic Component) (SNTA1))

Binding Specificity: AA 191-205, Intracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SNTA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Purpose: A Rabbit Polyclonal Antibody to α1-Syntrophin (SNTA1)

Specificity: Intracellular, PH1 domain

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-alpha1-Syntrophin (SNTA1) Antibody is directed against an epitope of mouse syntrophin alpha 1. Anti-α1-Syntrophin (SNTA1) Antibody (ABIN7043797, ABIN7045164 and ABIN7045165) can be used in western blot and immunohistochemistry applications. It has been designed to recognize syntrophin alpha 1 from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RQPSSPGPQPRNLSE, corresponding to amino acid residues 191-205 of mouse alpha1-syntrophin

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400

Application Dilutions Western blot wb: 1:200-1:400

Comment:

Negative Control: (ABIN7237350)

Blocking Peptide: (ABIN7237350)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SNTA1

Target: SNTA1 (Syntrophin, alpha 1 (Dystrophin-Associated Protein A1, 59kDa, Acidic Component) (SNTA1))

Alternative Name: SNTA1 (SNTA1 Products)

Background: Syntrophin alpha 1, Syntrophin-1, 59 kDa dystrophin-associated protein A1 acidic component 1,α1-Syntrophin is a component of the dystrophin scaffold-protein complex that acts in recruiting proteins through their multiple protein-protein interaction motifs to the cytoplasmic side of plasma membranes. Proteins interacting with α1-syntrophin include voltage-gated sodium channels, neuronal nitric oxide synthase, calmodulin, ankyrin repeat-rich membrane spanning protein, G-proteins and more. The syntrophin family comprises five members: α1-syntrophin, β1-syntrophin, β2-syntrophin, γ1-syntrophin, and γ2-syntrophin1.α1-Syntrophin is a peripheral cytoplasmic membrane protein in skeletal and cardiac muscle cells1.The α1-syntrophin structure contains four conserved domains, two pleckstrin homology domains (PH1 and PH2), a PSD95-disks large-ZO1 (PDZ) domain and a syntrophin unique (SU) COOH-terminal domain2. The pleckstrin domains are responsible for the recruitment of proteins to the sarcolemma. The PDZ domain is inserted within the PH1 domain and binds to NOS-1 in skeletal muscle. This domain also binds to the C-terminus of the Kir2.1 channel and also to NaV1.5 and so allowing them to interact. The SU domain binds syntrophin to dystrophin2,3. α1-Syntrophin is also responsible for the site-specific anchoring of AQP4 protein. α1-Syntrophin deletion leads to the disruption of the polarized subcellular expression of AQP4 in the perivascular membranes4. Several studies have shown that syntrophin-deficiency in Duchenne muscular dystrophy can ultimately lead to muscle degeneration. Syntrophin-deficiency was also shown to reduce levels of nNOS in mice5,6.

Alternative names: alpha1-Syntrophin (SNTA1), Syntrophin alpha 1, Syntrophin-1, 59 kDa dystrophin-associated protein A1 acidic component 1

Gene ID: 20648

NCBI Accession: NM_003098

UniProt: Q61234

Pathways: Synaptic Membrane, Skeletal Muscle Fiber Development