TACR1 antibody (Extracellular) (FITC)

Catalog No. ABIN7043805

This anti-TACR1 antibody is a Rabbit Polyclonal antibody detecting TACR1 in FACS and LCI. Suitable for Rat.

Quick Overview for TACR1 antibody (Extracellular) (FITC) (ABIN7043805)

Target: TACR1 (Tachykinin Receptor 1 (TACR1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TACR1 antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-TACR1 Antibody (FITC)

Binding Specificity: AA 180-194, Extracellular

Purpose: A Rabbit Polyclonal Antibody to Neurokinin 1 Receptor Conjugated to the Fluorescent Dye FITC

Specificity: 2nd extracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 13,15 amino acid residues identical

Characteristics: Anti-Neurokinin 1 Receptor (NK1R) (extracellular) Antibody (ABIN7043803, ABIN7045318 and ABIN7045319) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, indirect live cell flow cytometry, and live cell imaging applications. It has been designed to recognize NK1 from rat, mouse, and human samples. \nAnti-Neurokinin 1 Receptor (NK1R) (extracellular)-FITC Antibody (ABIN7043803, ABIN7045318 and ABIN7045319)-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CMIEWPEHPNRTYEK, corresponding to amino acid residues 180-194 of rat NK1 receptor

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: N/A

Comment:

Negative Control: (ABIN7582044)

Blocking Peptide: (ABIN7237370)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 μL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for TACR1

Target: TACR1 (Tachykinin Receptor 1 (TACR1))

Alternative Name: TACR1

Background: NK1 receptor, Tachykinin receptor 1, TACR1, Substance-P receptor, SPR,Substance P (SP), Neurokinin A (NKA) and Neurokinin B (NKB) are all peptides belonging to the Tachykinin protein family. These three peptides which demonstrate a quite heterogeneity in their distribution exert their effect via three receptors: Neurokinin 1-3 receptors, members of the G-protein coupled receptor superfamily. However, Neurokinin 1 Receptor (NK1) preferentially binds Substance P, Neurokinin 2 Receptor (NK2) to NKA and Neurokinin 3 Receptor (NK3) to NKB1.Neurokinin receptors are distinguished by their seven transmembrane domains, an extracellular N-terminus and a cytosolic C-terminal. An unusual property of these receptors is the presence of introns as part of their structural organization1-3. Tachykinin receptors undergo alternative splicing. For example, NK1 is detected with different C-terminal lengths. The longer receptor isoform is found in the brain whereas the truncated form is mostly detected in the periphery1,4.Due to the broad expression profile of tachykinin peptides, their respective receptors are also expressed in a similar fashion. NK1 is widely expressed in neurons endothelial cells, muscle and immune system cells. NK2 is broadly expressed in the periphery and its expression in the brain is quite restricted. NK3 on the other hand is largely expressed in the central nervous system and is also detected in the uterus, skeletal muscle, lung and liver1.Neurokinin receptors have been found in many pathophysiological indications and have therefore become targets for the development of pharmacological compounds. Such indications include cancer, psychological disorders, migraine and various inflammations, just to name a few5-8.

Alternative names: Neurokinin 1 Receptor (NK1R), NK1 receptor, Tachykinin receptor 1, TACR1, Substance-P receptor, SPR

Gene ID: 24807

NCBI Accession: NM_001058

UniProt: P14600

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour, Smooth Muscle Cell Migration