TRPM5 antibody (Intracellular)

Catalog No. ABIN7043834

Product Details anti-TRPM5 Antibody

Target: TRPM5 (Transient Receptor Potential Cation Channel, Subfamily M, Member 5 (TRPM5))

Binding Specificity: AA 32-45, Intracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRPM5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to TRPM5 Channel

Specificity: Intracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,human - 13,14 amino acid residues identical

Characteristics: Anti-TRPM5 Antibody (ABIN7043834, ABIN7044010 and ABIN7044011) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize TRPM5 from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Grade: KO Validated

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: GSGKKRGKFVKVPS(C), corresponding to amino acid residues 32-45 of mouse TRPM5

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IHC|ICC

Negative Control: (ABIN7237459)

Blocking Peptide: (ABIN7237459)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for TRPM5

Target: TRPM5 (Transient Receptor Potential Cation Channel, Subfamily M, Member 5 (TRPM5))

Alternative Name: TRPM5 (TRPM5 Products)

Background: LTrpC5, MTR1, Transient receptor potential cation channel subfamily M member 5,The mammalian melastatin-related transient receptor potential (TRPM) is a subfamily of the TRP family. The family was named after the first discovered member, melastatin (TRPM1) whose gene was identified in metastatic and benign melanomas1-3. The TRPM family consists of eight members designated as TRPM1-8 that can be further divided into four pairs: TRPM1 and TRPM3, TRPM2 and TRPM8, TRPM4 and TRPM5, and TRPM6 and TRPM71,2.The TRPM proteins share structural homology with other members of the TRP superfamily channels, six putative transmembrane domains, and cytoplasmic N- and C-termini. However, due to their long N- and C-termini they are also named the long TRP channel family1-3. TRPM4 and TRPM5 are the only TRP channels that are permeable to monovalent cations but not to Ca2+. TRPM4 is expressed as at least two alternative spliced isoforms, TRPM4a and TRPM4b which consist of shorter and longer N-terminal regions, respectively. TRPM4a displays little activity, TRPM4b and TRPM5 appear to be directly activated by an increase in intracellular Ca2+ concentration4,5.TRPM5 is primarily expressed in taste buds and also in stomach, small intestine, uterus and testes2. Regulation of TRPM5 by G-protein-coupled receptors and PLC-mediated IP3 production has been substantiated by the colocalization of TRPM5, of a taste-cell-specific PLC isoform and of receptors for sweet, bitter and amino acid (umami) tastes in taste tissue. It has furthermore been shown that desensitization of TRPM5 following receptor stimulation can be reversed by phosphatidylinositol (4,5)-bisphosphate (PIP2), the substrate of PLC2.

Alternative names: TRPM5, LTrpC5, MTR1, Transient receptor potential cation channel subfamily M member 5

Gene ID: 56843

NCBI Accession: NM_014555

UniProt: Q9JJH7