TNFRSF19 antibody (Extracellular)

Catalog No. ABIN7043868

Product Details anti-TNFRSF19 Antibody

Target: TNFRSF19 (Tumor Necrosis Factor Receptor Superfamily, Member 19 (TNFRSF19))

Binding Specificity: AA 75-88, Extracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNFRSF19 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunochromatography (IC), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to TROY

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,human - 13,14 amino acid residues identical

Characteristics: Anti-TROY (TNFRSF19) (extracellular) Antibody (ABIN7043868, ABIN7044792 and ABIN7044793) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, live cell imaging, and immunocytochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the protein in living cells. It has been designed to recognize TROY from human, mouse, and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CRPHRFKEDWGFQK, corresponding to amino acid residues 75-88 of mouse TROY

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:400

Comment:

Negative Control: (ABIN7237536)

Blocking Peptide: (ABIN7237536)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for TNFRSF19

Target: TNFRSF19 (Tumor Necrosis Factor Receptor Superfamily, Member 19 (TNFRSF19))

Alternative Name: TNFRSF19 (TNFRSF19 Products)

Background: TNF receptor superfamily member 19, Toxicity and JNK inducer, TAJ, TRADE,Tumor necrosis factor (TNF) is a potent mediator of inflammation as well as many normal physiological functions in homeostasis and health, and anti-microbial immunity.The TNF receptor superfamily member 19 (also known as TROY, TNFRSF19) is a type I cell surface transmembrane protein with disulfide bonds that form "cysteine-rich domains" (CRDs). These 40 amino acid pseudo-repeats are defined by 3 intra-chain disulfides generated by 6 highly conserved cysteines1. Unlike other members of the TNF receptor superfamily, TROY lacks a cytoplasmic death domain but contains a single TNF receptor-associated factor (TRAF)-binding site2. TROY is expressed on migrating or proliferating progenitor cells of the hippocampus, thalamus and cerebral cortex.TROY is involved in the pathogenesis of various neoplasms. Glioblastoma multiforme (GBM) is the most malignant form of all primary adult brain tumors in which patient survival remains approximately 1 year. Overexpression of TROY in glioma cells activates Rac1 signaling in a Pyk2-dependent manner to drive glioma cell invasion and migration. TROY expression has been found to correlate inversely with overall patient survival in glioblastoma3.TROY is also involved in the lack of successful axon regeneration in the adult central nervous system. Inhibition of axonal growth arises from inhibitory molecules in CNS myelin, which signal through a common receptor complex on neurons consisting of the ligand-binding Nogo-66 receptor (NgR) and two transmembrane co-receptors, p75NTR and LINGO-1. TROY can substitute p75 and form a tri-receptor complex with NgR and LINGO-1. This complex can reconstitute the activation of RhoA, a required step in the signaling pathways of myelin inhibition. Additionally, both overexpression of a truncated form of TROY lacking its intracellular domain and addition of a TROY-Fc fusion protein can block neuronal response to myelin inhibitors in a dominant-negative manner4.

Alternative names: TROY (TNFRSF19), TNF receptor superfamily member 19, Toxicity and JNK inducer, TAJ, TRADE

Gene ID: 29820

NCBI Accession: NM_018647

UniProt: Q9JLL3