TPCN1 antibody (AA 221-235)

Catalog No. ABIN7043871

Product Details anti-TPCN1 Antibody

Target: TPCN1 (Two Pore Segment Channel 1 (TPCN1))

Binding Specificity: AA 221-235

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TPCN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: A Rabbit Polyclonal Antibody to Two Pore Calcium Channel Protein 1

Specificity: 2nd luminal loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-TPCN1 Antibody (ABIN7043871 and ABIN7044036)) is a highly specific antibody directed against an epitope of the rat Two pore calcium channel protein 1. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize TPCN1 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RNLRQIFQSLPPFMD, corresponding to amino acid residues 221-235 of rat TPCN1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-CC071

Blocking Peptide: BLP-CC071

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for TPCN1

Target: TPCN1 (Two Pore Segment Channel 1 (TPCN1))

Alternative Name: TPCN1 (TPCN1 Products)

Background: Two pore calcium channel protein 1, Voltage-dependent calcium channel protein TPC1, Two pore segment channel 1,Among various vertebrate species, three genes are known to encode two-pore segment channels (TPCs) termed TPC1-3. Interestingly TPC3 seems to be absent from the genomes of primates and rodents1. The primary sequence of these channels indicates the presence of two putative pore-forming repeats. Each repeat contains six transmembrane domains and a pore loop, a structure strikingly reminiscent of many voltage-gated Na+ (Nav) and Ca2+ (Cav) channels2. These twelve transmembrane structures are further thought to form functional dimers3. Both TPC1 and TPC2 show ubiquitous expression, while that of TPC1 is exceptionally high in spleen, lung, liver, and kidney2.Ca2+-mobilizing messengers such as inositol triphosphate, cyclic ADP ribose and nicotinic acid adenosine dinucleotide phosphate (NAADP) are responsible for the intracellular changes in Ca2+ ion concentration4.In contrast to the other Ca2+-mobilizing agents, NAADP, the most potent of these Ca2+ releasing molecules increases the cytosolic Ca2+ concentration via Ca2+ channels located on acidic vesicles (endolysosomes)5,6. Only quite recently, after almost a decade of being cloned, TPC1 and TPC2 were both found to be responsible for the NAADP-induced release of Ca2+ 7,8. Evidence that these two channels are indeed responsible for the release of Ca2+ is quite compelling since overexpression of TPC1 and its knockdown or point mutation of a critical residue increase and exacerbate Ca2+ release respectively7. In addition, b-cells from TPC2 knockout mice exhibited no Ca2+ release from endolysosomes upon NAADP stimulation8. Finally, in a study using immunopurified channels, it was demonstrated that TPC1 and TPC2 both respond to very low concentrations of NAADP and are unequivocally responsible for the release of Ca2+, whereas TPC3 may negatively regulate the release of Ca2+ 9.As these channels have only recently been discovered, very little is known about their physiology and gating mechanisms. Their probable involvement in a number of diseases such as lysosomal storage disease (LSDs), caused by the dysfunction of lysosomal associated proteins, has yet to be deciphered1.

Alternative names: TPCN1, Two pore calcium channel protein 1, Voltage-dependent calcium channel protein TPC1, Two pore segment channel 1

Gene ID: 246215

NCBI Accession: NM_017901

UniProt: Q9WTN5