Recombinant CD28 antibody

Catalog No. ABIN7072236

This anti-CD28 antibody is a Mouse Monoclonal antibody detecting CD28 in FACS, IHC, SPR, CoSt and Agon. Suitable for Mouse.

Quick Overview for Recombinant CD28 antibody (ABIN7072236)

Target: CD28

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD28 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Surface Plasmon Resonance (SPR), Co-Stimulation (CoSt), Agonist (Agon)

Clone: D665

Product Details anti-CD28 Antibody

Purpose: Anti-CD28 [D665], Mouse IgG1, kappa

Specificity: This antibody binds to the laterally exposed C'D loop of CD28 and induces T cell proliferation without TCR ligation.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: The original D665 antibody was generated by immunizing mice alternately with A20 cells expressing mCD28 i.p. and with recombinant mCD28Ig s.c.

Isotype: IgG1 kappa

Application Details

Application Notes: This antibody binds to mouse CD28. The original mouse IgG1 version was shown to trigger T-cell proliferation in vitro and in vivo in a TCR-independent manner and is a superagonistic antibody. This antibody does not compete with E18 (Ab00286).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD28

Target: CD28

Alternative Name: CD28

Background: TP44, T-cell-specific surface glycoprotein CD28, TP44

UniProt: P31041

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response