Recombinant CD28 antibody

Catalog No. ABIN7072327

This Mouse Monoclonal antibody specifically detects CD28 in FACS, ELISA, Func, Act, BP and in vivo. It exhibits reactivity toward Dog.

Quick Overview for Recombinant CD28 antibody (ABIN7072327)

Target: CD28

Antibody Type: Recombinant Antibody

Reactivity: Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD28 antibody is un-conjugated

Application: Flow Cytometry (FACS), ELISA, Functional Studies (Func), Activation (Act), Blocking Peptide (BP), In vivo Studies (in vivo)

Clone: 1C6

Product Details anti-CD28 Antibody

Purpose: Anti-CD28 [1C6], Mouse IgG1, kappa

Specificity: The antibody binds specifically to canine CD28, a self-surface receptor expressed by thymocytes and a subset of natural killer cells. CD28 acts as a receptor for CD80 and CD86, providing a costimulatory signal for the activation of T-cells and the production of interleukins, such as IL-6.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: Canine CD28.

Isotype: IgG1 kappa

Application Details

Application Notes: This antibody exhibits an antagonistic activity, blocking mixed lymphocyte reactions and cytotoxic T lymphocyte-mediated killing. The use of this antibody to neutralize the CD26 signal following T-cell receptor activation is of clinical importance for transplantation therapy, as it has been shown to prevent graft rejection and graft-versus-host disease in the canine model of allogeneic hematopoietic cell transplantation (HCT). Interestingly, this antibody also exhibits agnostic activity, it was coated onto magnetic beads and used for the activation of canine peripheral blood lymphocytes (Szopa et al., 2021, PMID: 33679741).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD28

Target: CD28

Alternative Name: CD28

Background: TP44, Costimulatory molecule B7 receptor CD28, T-cell costimulatory molecule CD28

UniProt: Q9N0N8

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response