Recombinant CD16 antibody

Catalog No. ABIN7072393

This Rabbit Chimeric antibody specifically detects CD16 in FACS, IP, IHC, BR, Dep and SPR. It exhibits reactivity toward Human, Rhesus Monkey, Cynomolgus, Baboon, Capuchin Monkey, Chimpanzee, Macaca nemestrina, Marmoset, Saguinus oedipus, Sooty mangabey (Cercocebus atys) and Squirrel Monkey.

Quick Overview for Recombinant CD16 antibody (ABIN7072393)

Target: CD16

Antibody Type: Recombinant Antibody

Reactivity: Human, Rhesus Monkey, Cynomolgus, Baboon, Capuchin Monkey, Chimpanzee, Macaca nemestrina, Marmoset, Saguinus oedipus, Sooty mangabey (Cercocebus atys), Squirrel Monkey

Host: Rabbit

Clonality: Chimeric

Conjugate: This CD16 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Blocking Reagent (BR), Depletion (Dep), Surface Plasmon Resonance (SPR)

Clone: 3G8

Product Details anti-CD16 Antibody

Purpose: Anti-Fc gamma receptor III [3G8], Rabbit IgG, kappa

Specificity: 3G8 binds to CD16 at an epitope which is not the IgG-binding site. This antibody cannot distinguish between CD16a transmembrane isoform found on the surface of NK cells and macrophages (which can trigger the cytolytic response), and the GPI-linked CD16b isoform present on neutrophils or soluble CD16 found in the plasma. CD16 is the low affinity IgG receptor III and binds to aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis and ADCC (antibody-dependent cell-mediated cytotoxicity).

Cross-Reactivity: Baboon, Capuchin Monkey, Chimpanzee, Cynomolgus, Macaca nemestrina, Marmoset, Rhesus Monkey, Saguinus oedipus, Sooty mangabey (Cercocebus atys), Squirrel Monkey

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: 3G8 was prepared by immunizing mice with human PMN (polymorphonuclear leukocytes) cells.

Isotype: IgG kappa

Application Details

Application Notes: 3G8 antibody was used to construct a recombinant bispecific scFv (bsscFv) along with the mAb clone F3.3 anti-HLA class II antibody (connected by a (Gly4Ser)4 linker)which was expressed in SF21 insect cells. This bsscFv allows NK cells to target malignant B lymphoid cells and mediates effector cell lysis (ASCC) of the malignant cells (also lysis of primary leukaemia samples). 3G8 is also able to block neutrophil phagocytosis and stimulates NK cell proliferation, and can be used in FC, SPR, IP and IHC studies.

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD16

Target: CD16

Alternative Name: Fc gamma receptor III

Background: CD16, low affinity IgG receptor III, FcγRIII, low affinity FcγRIII

UniProt: Q9ULV2

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process