Recombinant Hemoglobin antibody

Catalog No. ABIN7072452

This anti-Hemoglobin antibody is a Rabbit Chimeric antibody detecting Hemoglobin in WB and ELISA. Suitable for Mouse.

Quick Overview for Recombinant Hemoglobin antibody (ABIN7072452)

Target: Hemoglobin

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Rabbit

Clonality: Chimeric

Conjugate: This Hemoglobin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 2A1

Product Details anti-Hemoglobin Antibody

Purpose: Anti-Haemoglobin [2A1], Rabbit IgG, kappa

Specificity: 2A1 exhibited polyreactive recognition of peptide 100-119 from the alpha subunit and peptide 100-119 frmo the beta subunit. Moreover, 2A1 bound Hb and the Hb-haptoglobin complex with equal efficiency, implying different binding sites on Hb for haptoglobin and 2A1. 2A1 was also found to distinguish between Hb and metHb.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG

Purification: Protein A affinity purified

Immunogen: Murine hybridomas were generated using spleen cells from aging (8-10 month old) NZB/W F1 animals. Hybridomas secreting antibodies reactive to murine hemoglobin were subcloned by limiting dilution and antibody isotypes determined using commercial reagents. Eight-week-old NZB/W F1 were immunized subcutaneously with 50 mg mHb antigen emulsified in Complete Fruend's Adjuvant.

Isotype: IgG kappa

Application Details

Application Notes: 2A1 has been used to bind to mHb in Western Blots and has been found to increase the secretion of TNF-alpha from endothelial cells when co-incubated with Hb, suggesting that 2A1 induces pro-inflammatory events (Bhatnagar 2011).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for Hemoglobin

Target: Hemoglobin

Alternative Name: Haemoglobin

Background: Hemoglobin, Hb

UniProt: P01942