Recombinant VISTA antibody

Catalog No. ABIN7072464

This anti-VISTA antibody is a Rabbit Chimeric antibody detecting VISTA in ELISA, IHC and BR. Suitable for Mouse.

Quick Overview for Recombinant VISTA antibody (ABIN7072464)

Target: VISTA (V-type immunoglobulin domain-containing suppressor of T-cell activation (VISTA))

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Rabbit

Clonality: Chimeric

Conjugate: This VISTA antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Blocking Reagent (BR)

Clone: MH5A

Product Details anti-VISTA Antibody

Purpose: Anti-PD-1H [MH5A], Rabbit IgG, lambda

Specificity: This antibody specifically binds PD-1H.

Characteristics:

Original Species of Ab: Hamster, Armenian

Original Format of Ab: IgG

Purification: Protein A affinity purified

Immunogen: The antibody was generated by immunising Armenian hamsters with mPD-1HIg.

Isotype: IgG lambda

Application Details

Application Notes: The antibody has been labelled with biotin and used to stain formalin-fixed tissues from wild-type C57BL/6 mice at 6 weeks of age. This showed specific staining for PD-1H in the T cell zone and marginal zone. The antibody stains P815 cells transfected with full-length PD-1H plasmid, but not control plasmid. Antibody specificity has been confirmed by ELISA. A single 200-μg dose of MH5A prevented aGVHD in mouse models, caused a reduction in accumulation and expansion of CD8+ and CD4+ T cells in spleens and livers and greatly reduced numbers of inflitrating T cells in all aGVHD target tissues examined. Few mAbs targeting a single cosignalling molecule have such a potent preventative effect (Flies D et al, 2011).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Hamster IgG format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for VISTA

Target: VISTA (V-type immunoglobulin domain-containing suppressor of T-cell activation (VISTA))

Alternative Name: PD-1H

Background: VISTA, VISR, B7-H5, B7H5, GI24, PP2135, SISP1, DD1alpha, C10orf54, chromosome 10 open reading frame 54, V-set immunoregulatory receptor

UniProt: Q9D659

Pathways: Cancer Immune Checkpoints