Recombinant CD40 antibody

Catalog No. ABIN7072517

The Rabbit Chimeric anti-CD40 antibody has been validated for FACS, IHC and IP. It is suitable to detect CD40 in samples from Mouse.

Quick Overview for Recombinant CD40 antibody (ABIN7072517)

Target: CD40

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Rabbit

Clonality: Chimeric

Conjugate: This CD40 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Clone: 3-23

Product Details anti-CD40 Antibody

Purpose: Anti-CD40 [3/23], Rabbit IgG, kappa

Specificity: This antibody is specific for murine CD40, a 48 kD type I transmembrane glycoprotein also known as Bp50. It is a member of the tumor necrosis factor receptor (TNFR) superfamily and is expressed on B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, and a subset of CD34+ hematopoietic progenitors. CD40 regulates B cell development/maturation, Ig isotype switching and, in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with its ligand CD154 (gp39), which is expressed on activated T cells, is important in costimulation and immune regulation.

Characteristics:

Original Species of Ab: Rat

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: This antibody was raised by immunising Lou rats with murine CD40-hgamma1-Fc according to the protocol by Hasbold et al. (Eur J Immunol. 1994)

Isotype: IgG kappa

Application Details

Application Notes: This antibody has been used in various FACS analyses, for instance, to determine the immunoregulatory properties of primary colonic epithelial cells (Telega et al., 2000), to validate if eicosapentaenoic acid induces prolonged survival of cardiac allografts and generates regulatory T cells (Iwami et al., 2009), to characterise Siglec-H as a novel endocytic receptor expressed on murine plasmacytoid dendritic cell precursors (Zhang et al., 2006), to delineate the mechanisms for the activation of dendritic cells and induction of T cell responses by HPV 16 L1/E7 chimeric virus-like particles (Freyschmidt et al., 2004), and to investigate the role of epigenetic modification and immunomodulation in a murine prostate cancer model (Sulek et al., 2017). In a study by White et al. (J Immunol. 2011), the parental anti-mouse CD40 3/23 (rat IgG2a) mAb was first reformatted to mouse IgG1 (m1) and IgG2a (m2a) versions. The new variants were then utilised in immunohistochemistry, SPR, and in vitro phagocytosis assays and assessments for B cell proliferation, activation, and survival to confirm that interaction with FcγRIIB is critical for the agonistic activity of anti-CD40 amAb (White et al. 2011).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD40

Target: CD40

Alternative Name: CD40

Background: TNFRSF5, B-cell surface antigen CD40, Bp50, CD40, CD40L receptor, CDW40, MGC9013, T-cell differentiation antigen, Tumor necrosis factor receptor superfamily member 5, tumor necrosis factor receptor superfamily, member 5

UniProt: P27512

Pathways: NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints