Recombinant RANKL antibody

Catalog No. ABIN7072529

The Rabbit Chimeric anti-RANKL antibody has been validated for IHC, FACS and Neut. It is suitable to detect RANKL in samples from Mouse.

Quick Overview for Recombinant RANKL antibody (ABIN7072529)

Target: RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Rabbit

Clonality: Chimeric

Conjugate: This RANKL antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Neutralization (Neut)

Clone: IK22-5

Product Details anti-RANKL Antibody

Purpose: Anti-RANKL [IK22-5], Rabbit IgG, kappa

Specificity: This antibody is specific for murine RANKL.

Characteristics:

Original Species of Ab: Rat

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: Thus antibody was raised by immunising an SD rat with murine RANKL-transfected baby hamster kidney cells.

Isotype: IgG kappa

Application Details

Application Notes: This antibody has been used in immunohistochemical analysis of frozen Peyer's patch tissue sections (Kobayashi et al, 2013). It has also been used in flow cytometric analysis of RANKL cell surface expression by Th1 and Th17 cells (Nakae et al, 2007), RANKL-transfected human embryonic kidney cells (Miyahira et al, 2003) and murine T hybridoma 2B4.11 cells (Fionda et al, 2007). Additionally, this RANKL-neutralising antibody has been used to assess the effects of RANKL inhibition on myoepithelial cell proliferation in mammary organoid culture (Haslam et al, 2008). Treatment of collagen-induced arthritis (CIA) mice with this antibody has been shown to reduce bone loss at the site of inflammation (Kamijo et al, 2006), while administration to C57BL/6J mice induced transient tibia and skull bone growth arrest and irreversible blockage of tooth eruption (Lezot et al, 2015).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for RANKL

Target: RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))

Alternative Name: RANKL

Background: CD254, IK22/5, RANK Ligand, Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, ODF, Osteoprotegerin ligand, OPGL, Receptor activator of nuclear factor kappa-B ligand, TNF-related activation-induced cytokine, TRANCE

UniProt: O35235

Pathways: NF-kappaB Signaling