Recombinant ITGAL antibody

Catalog No. ABIN7072544

The Mouse Monoclonal anti-ITGAL antibody has been validated for InhA. It is suitable to detect ITGAL in samples from Human.

Quick Overview for Recombinant ITGAL antibody (ABIN7072544)

Target: ITGAL (Integrin alpha-L (ITGAL))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITGAL antibody is un-conjugated

Application: Inhibition Assay (InhA)

Clone: MHM24

Product Details anti-ITGAL Antibody

Purpose: Anti-CD11a [MHM24], Mouse IgG1, kappa

Specificity: MHM24 binds to the CD11a subunit of lymphocyte function-associated antigen. It binds to both the high- and low-affinity forms of the antigen.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG

Purification: Protein A affinity purified

Immunogen: Spleen cells from BALB/c mice immunised with EBV-transformed human lymphoblastoid cells were fused with P3-NSUlAg4.1 myeloma cells and the resulting hybridomas were screened for antibody production by a radioimmunoassay on T and B cell lines.

Isotype: IgG1 kappa

Application Details

Application Notes: Cytofluorography was used to show the binding of MHM24 to peripheral blood lymphocytes, with 97.3 % of cells stained by MHM24 (Hildreth et al, 1983). In inhibition experiments, MHM24 inhibited T cell-mediated lysis of virus-infected cells (Hildreth et al, 1983). Additionally, MHM24 inhibited NK cell-mediated lysis (Hildreth et al, 1983).Flow cytometry showed MHM24 exhibits non-selective binding to high-affinity and low-affinity forms of LFA-1 (Huang et al, 2006). Murine MHM24 was humanised and shown to prevent adhesion of human T cells to human keratinocytes and the proliferation of T cells in response to nonautologous leukocytes (Werther et al, 1996).

Comment:

This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for ITGAL

Target: ITGAL (Integrin alpha-L (ITGAL))

Alternative Name: CD11a

Background: Integrin alpha-L, CD11 antigen-like family member A, leukocyte function-associated molecule 1 alpha chain, human lymphocyte function antigen

UniProt: P20701

Pathways: Activated T Cell Proliferation, Integrin Complex