Recombinant gamma-Aminobutyric Acid Receptor Subunit beta (RDL) antibody

Catalog No. ABIN7072589

The Rabbit Chimeric anti- antibody has been validated for Crys and SEC-HPLC. It is suitable to detect in samples from Human.

Quick Overview for Recombinant gamma-Aminobutyric Acid Receptor Subunit beta (RDL) antibody (ABIN7072589)

Target: gamma-Aminobutyric Acid Receptor Subunit beta (RDL)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: Un-conjugated

Application: Crystallization (Crys), Size-exclusion chromatography-High Pressure Liquid Chromatography (SEC-HPLC)

Clone: 1F4

Product Details

Purpose: Anti-GABAR [1F4], Rabbit IgG, kappa

Specificity: This antibody recognises α1β2γ2 GABA-A receptor which is found in up to 50 % of the synapses in the brain. It's ligand, GABA is a major inhibitory transmitter in the nervous system, binds to this receptor and opens a channel permeable to chloride. Disfunctions of GABAR are found in anxiety, neurodevelopmental disorderds and epilepsy.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2b

Purification: Protein A affinity purified

Immunogen: This antibody was raised against alpha1beta2gamma2 GABA-A receptor.

Isotype: IgG kappa

Application Details

Application Notes: FSEC: High affinity and specificity of the 1F4 antibody to α1β2γ2 GABA-A receptor was demonstrated using fluorescence-detection size exclusion chromatography with GFP-tagged receptor (shift in elution volume) (Zhu et al., 2018). Crystallography: Furthermore, this antibody was utilised in production and purification of the α1β2γ2 GABA-A receptor (Zhu et al., 2018). Finally, Fab fragments derived from the antibody were used to facilitate crystallisation and improve stabilisation of the α1β2γ2 GABA-A receptor (Zhu et al., 2018).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details

Target: gamma-Aminobutyric Acid Receptor Subunit beta (RDL)

Alternative Name: GABAR

Background: Gamma-aminobutyric acid receptor A, Gamma-aminobutyric acid receptor, Gamma-aminobutyric acid receptor subunit alpha-1, Gamma-aminobutyric acid receptor subunit beta-2, Gamma-aminobutyric acid receptor subunit gamma-2, GABA(A), GABA-A receptor, α1β2γ2 GABA-A receptor

UniProt: P14867, P47870, P18507