Recombinant Lewis Blood Group A/b antibody
Reactivity: Human
FACS, IF, IHC, IP, WB
Host: Rabbit
Chimeric
HEA164
unconjugated
Recombinant Antibody
-
- Target
- Lewis Blood Group A/b
- Antibody Type
- Recombinant Antibody
- Reactivity
- Human
-
Host
- Rabbit
-
Clonality
- Chimeric
-
Conjugate
- Un-conjugated
- Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
- Purpose
- Anti-Lewis a,b [HEA164], Rabbit IgG, kappa
- Specificity
- This antibody is specific for Lewis a,b carbohydrate antigens.
- Characteristics
-
Original Species of Ab: Mouse
Original Format of Ab: IgM
- Purification
- Protein A affinity purified
- Immunogen
- This antibody was raised by immunising mice with human carcinoma cells.
- Clone
- HEA164
- Isotype
- IgG kappa
-
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- Application Notes
- This antibody has applications in the detection of normal and neoplastic epithelial cells. This antibody has been shown to react with a glycolipid fraction isolated from colon carcinoma tissue, with no effect of pronase E addition on its reactivity (Moldenhauer et al, 1987). This antibody, when immobilised on the wall of a shear chamber, has been shown to support slow leukocyte rolling (Chen et al, 1997).
- Comment
-
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgM format, for improved compatibility with existing reagents, assays and techniques.
- Restrictions
- For Research Use only
-
- Concentration
- 1 mg/mL
- Buffer
- PBS with 0.02 % Proclin 300.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
-
- Target
- Lewis Blood Group A/b
- Alternative Name
- Lewis a,b
- Background
- Blood Group Lewis a/b, lewis a, lewis b
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