Recombinant CCR5 antibody (pSer349)

Catalog No. ABIN7072657

Product Details anti-CCR5 Antibody

Target: CCR5 (Chemokine (C-C Motif) Receptor 5 (CCR5))

Antibody Type: Recombinant Antibody

Binding Specificity: pSer349

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This CCR5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Purpose: Anti-CCR5 (phosphoserine 349) [E11/19], Rabbit IgG, kappa

Specificity: The E11/19 monoclonal antibody binds to phosphoserine 349 corresponding to 22 c-terminal amino acid residue of human CCR5. CCR5 is a receptor for a number of inflammatory CC-chemokines including CCL3/MIP-1-alpha, CCL4/MIP-1-beta and RANTES.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: This antibody was raised by immunizing mice with a synthetic phosphopeptide (CEAPERA(pS)(pS)VYTR(pS)TGEQI(pS)VGL) corresponding to the C-terminal region of human CCR5.

Clone: E11-19

Isotype: IgG kappa

Application Details

Application Notes: The E11/19 antibody is recommended for for flow cytometric analysis, immunoblotting, and ELISA assay of CCR5 expression. It was also successfully utilized in the immunoprecipitation of CCR5 (Venuti et al., 2016). This antibody was demonstrated to detect pS349 CCR5 via Western blot when it was first activated to its phosphorylated form (Pollok-Kopp et al., 2003). Furthermore, E11/19 antibody was used to identify the localization of pS349 CCR5 in RBL cells via immunofluorescence (Pollok-Kopp et al., 2003). With other anti-CCR5 antibodies which react with nonphosporylated CCR5 (Ab01577) or CCR5 phosphorylated on different serine residues (Ab01579), it can be used to study dynamic regulation of CCR5 and of phosphorylation mechanisms.

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CCR5

Target: CCR5 (Chemokine (C-C Motif) Receptor 5 (CCR5))

Alternative Name: CCR5 (CCR5 Products)

Background: CD195, C-C chemokine receptor type 5, C-C CKR-5, CC-CKR-5, CCR-5, CHEMR13, HIV-1 fusion coreceptor, p349, pS349, Ser-349, Ser349

UniProt: P51681

Pathways: Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Regulation of Cell Size