Recombinant Histone H4 antibody (acLys8, acLys12, acLys16)

Catalog No. ABIN7072679

The Rabbit Chimeric anti-Histone H4 antibody has been validated for WB, IHC, IF and ELISA. It is suitable to detect Histone H4 in samples from Human.

Quick Overview for Recombinant Histone H4 antibody (acLys8, acLys12, acLys16) (ABIN7072679)

Target: Histone H4

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This Histone H4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Clone: KM-2

Product Details anti-Histone H4 Antibody

Binding Specificity: acLys8, acLys12, acLys16

Purpose: Anti-H4 K8Ac, K12Ac, K16Ac [KM-2], Rabbit IgG, kappa

Specificity: KM-2 antibody recognizes the N-terminal peptides 1-20 of histone H2A and 1-29 of histone H4 which have a large sequence homology (van Bruggen et al., 1997, pmid: 9027774). Histone H4 acetylation on lysines 8, 12 and 16 significantly increases the antibody's binding while the acetylation at K5 decreases it (Dieker et al., 2007, pmid: 17530637).

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: KM-2 antibody was derived from an MRL/lpr lupus mouse.

Isotype: IgG kappa

Application Details

Application Notes: The antibody KM-2 shows significant increase in activity with histone H4 when it is acethylated on lysines 8, 12 and 16 which are hyperacethylated in conditions, such as lupus (SLE), and thus KM-2 can be used in the detection of the apoptosis-induced histone modifications characteristic of lupus and in the analysis of its pathogenesis (Dieker et al., 2007, pmid: 17530637). This antibody was successfully used to identify both Histone H4 as well as H2A in the extracts of Jurkat cells via Western blot, showing considerable increase in activity in cells with induced apoptosis leading to hyperacethylation (Dieker et al., 2007, pmid: 17530637). Similar results were achieved for the immunofluorescence staining of Jurkat cells. The enhanced reactivity of KM-2 with apoptotic H4 and apoptotic H2A was not Jurkat cell specific and was also observed for the monocytic U937 cell line which indicated its general applicability (Dieker et al., 2007, pmid: 17530637). Analogous reselts were also achieved during the ELISA analysis of plasma samples of (diseased) MRL/lpr lupus mice, with the visisble activity enhancement in the sick, apoptotic specimens (Dieker et al., 2007, pmid: 17530637). Finally, KM-2 (KM2) antibody was used to identify histones in the immune deposits in kidney biopsy samples of DPGN (Diffuse proliferative glomerulonephritis) patients (van Bruggen et al., 1997, pmid: 9027774).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for Histone H4

Target: Histone H4

Background: Histone 4, nucleosome, H4K8Ac, H4K12Ac, H4K16Ac, acetyl-Histone H4, Histone H4 acetylated at lysine, KM2