H2-AB1 antibody

Catalog No. ABIN7074228

The Rabbit Polyclonal anti-H2-AB1 antibody is suitable to detect H2-AB1 in samples from Mouse and Rat. It has been validated for IF and IHC.

Quick Overview for H2-AB1 antibody (ABIN7074228)

Target: H2-AB1 (Histocompatibility 2, Class II Antigen A, beta 1 (H2-AB1))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This H2-AB1 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (IHC)

Product Details anti-H2-AB1 Antibody

Cross-Reactivity: Rat

Purification: Affinity purification

Immunogen: Recombinant protein corresponding to Mouse H2ab1

Application Details

Application Notes: IHC/IF (M,R) 1:4000-1:8800

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for H2-AB1

Target: H2-AB1 (Histocompatibility 2, Class II Antigen A, beta 1 (H2-AB1))

Alternative Name: H2ab1

Background: Atypical histone H2A which replaces conventional H2A during late spermatogenesis and is involved in the replacement of histones to protamine in male germ cells . Core component of nucleosome: nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template . Nucleosomes containing H2AB1 only wrap 130 bp of DNA, compared to 147 bp for classical nucleosomes . In condensing spermatids, the heterodimer between H2AB1 and H2BC1/TH2B is loaded onto the nucleosomes and promotes loading of transition proteins (TNP1 and TNP2) onto the nucleosomes . Inclusion of the H2AB1-H2BC1/TH2B dimer into chromatin opens the nucleosomes, releasing the nucleosomal DNA ends and allowing the invasion of nucleosomes by transition proteins (TNP1 and TNP2) .

Gene ID: 68231

NCBI Accession: NP_080903

UniProt: Q9CQ70