alpha Actinin 4 antibody

Catalog No. ABIN7090722

This Rabbit Monoclonal antibody specifically detects alpha Actinin 4 in WB, IF, IHC (p) and ICC. It exhibits reactivity toward Human and Mouse.

Quick Overview for alpha Actinin 4 antibody (ABIN7090722)

Target: alpha Actinin 4 (ACTN4) (Actinin, alpha 4 (ACTN4))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This alpha Actinin 4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Clone: A12H9

Product Details anti-alpha Actinin 4 Antibody

Cross-Reactivity: Human, Mouse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human alpha Actinin 4

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IF()
ICC 1:100-500

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for alpha Actinin 4

Target: alpha Actinin 4 (ACTN4) (Actinin, alpha 4 (ACTN4))

Alternative Name: alpha Actinin 4

Background:

Synonyms: actinin 4, Actinin alpha 4, Actinin alpha-4, Alpha-actinin-4, _-Actinin-4, _Actinin-4, actinin4, ACTN 4, ACTN4, ACTN4_HUMAN, DKFZp686K23158, F actin cross linking protein, F-actin cross-linking protein, Focal segmental glomerulosclerosis 1, FSGS 1, FSGS, FSGS1, Non muscle alpha actinin 4, Non-muscle alpha-actinin 4.

Background: Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq, Jul 2008].

Gene ID: 81

UniProt: O43707

Pathways: Proton Transport