ATF4 antibody

Catalog No. ABIN7111244

The Rabbit Polyclonal anti-ATF4 antibody has been validated for WB, IHC, ELISA, IP and FACS. It is suitable to detect ATF4 in samples from Human, Mouse and Rat.

Quick Overview for ATF4 antibody (ABIN7111244)

Target: ATF4 (Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATF4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS)

Product Details anti-ATF4 Antibody

Purpose: ATF4 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: activating transcription factor 4(tax-responsive enhancer element B67)

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:1000, IP: 1:200-1:1000, IHC: 1:50-1:500, IF: 1:50-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for ATF4

Target: ATF4 (Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4))

Alternative Name: ATF4

Background:

Synonyms: Cyclic AMP-dependent transcription factor ATF-4 (cAMP-dependent transcription factor ATF-4)|Activating transcription factor 4|Cyclic AMP-responsive element-binding protein 2 (CREB-2, cAMP-responsive element-binding protein 2)|Tax-responsive enhancer element-binding protein 67 (TaxREB67)|ATF4|CREB2|TXREB

Background: Transcriptional activator. Binds the cAMP response element(CRE)(consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Cooperates with FOXO1 in osteoblasts to regulate glucose homeostasis through suppression of beta-cell production and decrease in insulin production(By similarity). It binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I. Regulates the induction of DDIT3/CHOP and asparagine synthetase(ASNS) in response to endoplasmic reticulum(ER) stress. In concert with DDIT3/CHOP, activates the transcription of TRIB3 and promotes ER stress-induced neuronal apoptosis by regulating the transcriptional induction of BBC3/PUMA. Activates transcription of SIRT4. Regulates the circadian expression of the core clock component PER2 and the serotonin transporter SLC6A4. Binds in a circadian time-dependent manner to the cAMP response elements(CRE) in the SLC6A4 and PER2 promoters and periodically activates the transcription of these genes. During ER stress response, activates the transcription of NLRP1, possibly in concert with other factors(PubMed:26086088).

Molecular Weight: 50-58 kDa, 35-38 kDa

Gene ID: 468

UniProt: P18848

Pathways: Thyroid Hormone Synthesis, Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Unfolded Protein Response