ALKBH7 antibody

Catalog No. ABIN7111484

The Rabbit Polyclonal anti-ALKBH7 antibody has been validated for WB, ELISA and IHC. It is suitable to detect ALKBH7 in samples from Human, Mouse and Rat.

Quick Overview for ALKBH7 antibody (ABIN7111484)

Target: ALKBH7 (AlkB, Alkylation Repair Homolog 7 (ALKBH7))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALKBH7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-ALKBH7 Antibody

Purpose: ALKBH7 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: alkB, alkylation repair homolog 7(E. coli)

Isotype: IgG

Application Details

Application Notes: WB: 1:200-1:2000, IHC: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for ALKBH7

Target: ALKBH7 (AlkB, Alkylation Repair Homolog 7 (ALKBH7))

Alternative Name: ALKBH7

Background:

Synonyms: Alpha-ketoglutarate-dependent dioxygenase alkB homolog 7, mitochondrial|Alkylated DNA repair protein alkB homolog 7|Spermatogenesis cell proliferation-related protein|Spermatogenesis-associated protein 11|ALKBH7|ABH7|SPATA11

Background: May function as protein hydroxylase, can catalyze auto-hydroxylation at Leu-110(in vitro), but this activity may be due to the absence of the true substrate(PubMed:25122757). Required to induce programmed necrosis in response to DNA damage caused by cytotoxic alkylating agents. Acts by triggering the collapse of mitochondrial membrane potential and loss of mitochondrial function that leads to energy depletion and cell death(PubMed:23666923). ALKBH7-mediated necrosis is probably required to prevent the accumulation of cells with DNA damage(PubMed:23666923). Does not display DNA demethylase activity(PubMed:23666923). Involved in fatty acid metabolism(By similarity).

Molecular Weight: 25 kDa

Gene ID: 84266

UniProt: Q9BT30