CREB3 antibody

Catalog No. ABIN7112240

The Rabbit Polyclonal anti-CREB3 antibody has been validated for WB, ELISA, IHC and IP. It is suitable to detect CREB3 in samples from Human.

Quick Overview for CREB3 antibody (ABIN7112240)

Target: CREB3 (CAMP Responsive Element Binding Protein 3 (CREB3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CREB3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-CREB3 Antibody

Purpose: CREB3 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: cAMP responsive element binding protein 3

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IP: 1:200-1:1000, IHC: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for CREB3

Target: CREB3 (CAMP Responsive Element Binding Protein 3 (CREB3))

Alternative Name: CREB3

Background:

Synonyms: Cyclic AMP-responsive element-binding protein 3 (CREB-3, cAMP-responsive element-binding protein 3)|Leucine zipper protein|Luman|Transcription factor LZIP-alpha|Processed cyclic AMP-responsive element-binding protein 3 (N-terminal Luman, Transcriptionally active form)|CREB3|LZIP

Background: Endoplasmic reticulum(ER)-bound transcription factor that plays a role in the unfolded protein response(UPR). Involved in cell proliferation and migration, tumor suppression and inflammatory gene expression. Plays also a role in the human immunodeficiency virus type 1(HIV-1) virus protein expression and in the herpes simplex virus-1(HSV-1) latent infection and reactivation from latency. Isoform 2 plays a role in the unfolded protein response(UPR). Isoform 2 acts as a positive regulator of LKN-1/CCL15-induced chemotaxis signaling of leukocyte cell migration. Isoform 2 may play a role as a cellular tumor suppressor that is targeted by the hepatitis C virus(HSV) core protein. Isoform 2 represses the VP16-mediated transactivation of immediate early genes of the HSV-1 virus by sequestring host cell factor-1 HCFC1 in the ER membrane of sensory neurons, thereby preventing the initiation of the replicative cascade leading to latent infection. Isoform 3 functions as a negative transcriptional regulator in ligand-induced transcriptional activation of the glucocorticoid receptor NR3C1 by recruiting and activating histone deacetylases(HDAC1, HDAC2 and HDAC6). Isoform 3 decreases the acetylation level of histone H4. Isoform 3 does not promote the chemotactic activity of leukocyte cells. Processed cyclic AMP-responsive element-binding protein 3: acts as a transcription factor that activates unfolded protein response(UPR) target genes during endoplasmic reticulum(ER) stress response. Promotes cell survival against ER stress-induced apoptotic cell death during UPR. Activates transcription from CRE and C/EBP-containing reporter genes. Induces transcriptional activation of chemokine receptors. Activates transcription of genes required for reactivation of the latent HSV-1 virus. Down-regulates Tat-dependent transcription of the HIV-1 LTR by interacting with HIV-1 Tat. It's transcriptional activity is inhibited by CREBZF in a HCFC1-dependent manner, by the viral transactivator protein VP16 and by the HCV core protein. Binds DNA to the cAMP response element(CRE)(consensus: 5'-GTGACGT[AG][AG]-3') and C/EBP sequences present in many viral and cellular promoters. Binds to the unfolded protein respons element(UPRE) consensus sequences sites. Binds DNA to the 5'-CCAC[GA]-3'half of ERSE II(5'-ATTGG-N-CCACG-3'). Associates with chromatin to the HERPUD1 promoter.

Molecular Weight: 40-64 kDa

Gene ID: 10488

UniProt: O43889

Pathways: Thyroid Hormone Synthesis, Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Maintenance of Protein Location, Unfolded Protein Response