Cullin 4A antibody (AA 460-759)

Catalog No. ABIN7113117

The Mouse Monoclonal anti-Cullin 4A antibody (Clone 5A7) (ABIN7113117) specifically detects Cullin 4A in WB, ELISA, IHC, IF and IP. The antibody is reactive with Human, Mouse, Rat and Monkey samples.

Quick Overview for Cullin 4A antibody (AA 460-759) (ABIN7113117)

Target: Cullin 4A (CUL4A)

Reactivity: Human, Mouse, Rat, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cullin 4A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 5A7

Product Details anti-Cullin 4A Antibody

Binding Specificity: AA 460-759

Purpose: CUL4A antibody

Purification: Protein A+G purification

Purity: ≥95 % as determined by SDS-PAGE

Immunogen:

Immunogen sequence: 460-759aa

Immunogen: cullin 4A

Isotype: IgG1

Application Details

Application Notes: WB: 1:500-1:2000, IP: 1:500-1:1000, IHC: 1:20-1:200, IF: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for Cullin 4A

Target: Cullin 4A (CUL4A)

Alternative Name: CUL4A

Background:

Synonyms: Cullin-4A (CUL-4A)|CUL4A

Background: Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. DCX(DDB2) ubiquitinates histones H3-H4 and is required for efficient histone deposition during replication-coupled(H3.1) and replication-independent(H3.3) nucleosome assembly, probably by facilitating the transfer of H3 from ASF1A/ASF1B to other chaperones involved in histone deposition. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. In association with DDB1 and SKP2 probably is involved in ubiquitination of CDKN1B/p27kip. Is involved in ubiquitination of HOXA9. DCX(DTL) directs autoubiquitination of DTL.

Molecular Weight: 88 kDa, 77 kDa

Gene ID: 8451

UniProt: Q13619