Cullin 4A antibody (AA 460-759)

Catalog No. ABIN7113117

This anti-Cullin 4A antibody is a Mouse Monoclonal antibody detecting Cullin 4A in WB, ELISA, IHC, IF and IP. Suitable for Human, Mouse, Rat and Monkey.

Quick Overview for Cullin 4A antibody (AA 460-759) (ABIN7113117)

Target: Cullin 4A (CUL4A)

Reactivity: Human, Mouse, Rat, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cullin 4A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 5A7

Product Details anti-Cullin 4A Antibody

Binding Specificity: AA 460-759

Purpose: CUL4A antibody

Purification: Protein A+G purification

Purity: ≥95 % as determined by SDS-PAGE

Immunogen:

Immunogen sequence: 460-759aa

Immunogen: cullin 4A

Isotype: IgG1

Application Details

Application Notes: WB: 1:500-1:2000, IP: 1:500-1:1000, IHC: 1:20-1:200, IF: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for Cullin 4A

Target: Cullin 4A (CUL4A)

Alternative Name: CUL4A

Background:

Synonyms: Cullin-4A (CUL-4A)|CUL4A

Background: Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. DCX(DDB2) ubiquitinates histones H3-H4 and is required for efficient histone deposition during replication-coupled(H3.1) and replication-independent(H3.3) nucleosome assembly, probably by facilitating the transfer of H3 from ASF1A/ASF1B to other chaperones involved in histone deposition. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. In association with DDB1 and SKP2 probably is involved in ubiquitination of CDKN1B/p27kip. Is involved in ubiquitination of HOXA9. DCX(DTL) directs autoubiquitination of DTL.

Molecular Weight: 88 kDa, 77 kDa

Gene ID: 8451

UniProt: Q13619