EIF3S1 antibody

Catalog No. ABIN7113950

The Rabbit Polyclonal anti-EIF3S1 antibody has been validated for WB, ELISA, IHC, IF and IP. It is suitable to detect EIF3S1 in samples from Human, Mouse and Rat.

Quick Overview for EIF3S1 antibody (ABIN7113950)

Target: EIF3S1 (Eukaryotic Translation Initiation Factor 3 Subunit J (EIF3S1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF3S1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-EIF3S1 Antibody

Purpose: EIF3J antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: eukaryotic translation initiation factor 3, subunit J

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IP: 1:200-1:1000, IHC: 1:20-1:200, IF: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for EIF3S1

Target: EIF3S1 (Eukaryotic Translation Initiation Factor 3 Subunit J (EIF3S1))

Alternative Name: EIF3J

Background:

Synonyms: Eukaryotic translation initiation factor 3 subunit J (eIF3j)|Eukaryotic translation initiation factor 3 subunit 1|eIF-3-alpha|eIF3 p35|EIF3J|EIF3S1

Background: Component of the eukaryotic translation initiation factor 3(eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex(43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. This subunit binds directly within the mRNA entry channel of the 40S ribosome to the aminoacyl(A) site. It may regulate the interaction between the 43S PIC and mRNA.

Molecular Weight: 35 kDa

Gene ID: 8669

UniProt: O75822

Pathways: Ribonucleoprotein Complex Subunit Organization