FBXO9 antibody

Catalog No. ABIN7114056

This anti-FBXO9 antibody is a Rabbit Polyclonal antibody detecting FBXO9 in WB, ELISA and IHC. Suitable for Human, Mouse and Rat.

Quick Overview for FBXO9 antibody (ABIN7114056)

Target: FBXO9 (F-Box Protein 9 (FBXO9))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FBXO9 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-FBXO9 Antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: F-box protein 9

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IHC: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for FBXO9

Target: FBXO9 (F-Box Protein 9 (FBXO9))

Alternative Name: FBXO9

Background: Synonyms:FBX9, VCIA1 Background:Substrate recognition component of a SCF(SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of TTI1 and TELO2 in a CK2-dependent manner, thereby directly regulating mTOR signaling. SCF(FBXO9) recognizes and binds mTORC1-bound TTI1 and TELO2 when they are phosphorylated by CK2 following growth factor deprivation, leading to their degradation. In contrast, the SCF(FBXO9) does not mediate ubiquitination of TTI1 and TELO2 when they are part of the mTORC2 complex. As a consequence, mTORC1 is inactivated to restrain cell growth and protein translation, while mTORC2 is activated due to the relief of feedback inhibition by mTORC1.

Molecular Weight: 52 kDa

Gene ID: 26268

UniProt: Q9UK97