HNRNPC antibody

Catalog No. ABIN7114978

This Rabbit Polyclonal antibody specifically detects HNRNPC in WB, IHC, ELISA, IF and FACS. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for HNRNPC antibody (ABIN7114978)

Target: HNRNPC (Heterogeneous Nuclear Ribonucleoprotein C (C1/C2) (HNRNPC))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HNRNPC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-HNRNPC Antibody

Purpose: HNRNPC antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: heterogeneous nuclear ribonucleoprotein C(C1/C2)

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IHC: 1:20-1:200, IF: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for HNRNPC

Target: HNRNPC (Heterogeneous Nuclear Ribonucleoprotein C (C1/C2) (HNRNPC))

Alternative Name: HNRNPC

Background:

Synonyms: Heterogeneous nuclear ribonucleoproteins C1/C2 (hnRNP C1/C2)|HNRNPC|HNRPC

Background: Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles(PubMed:8264621). Specifically recognizes and binds N6-methyladenosine(m6A)-containing RNAs, a modification present at internal sites of mRNAs that affects mRNA splicing, processing and stability. M6A alters the local structure in mRNAs and long non-coding RNAs(lncRNAs) via a mechanism named 'm(6)A-switch' to facilitate binding of HNRNPC, leading to regulation of mRNA splicing(PubMed:25719671). Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides. May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules(PubMed:12509468, PubMed:16010978, PubMed:7567451, PubMed:8264621).

Molecular Weight: 32 kDa

Gene ID: 3183

UniProt: P07910