ISCU antibody

Catalog No. ABIN7115516

The Rabbit Polyclonal anti-ISCU antibody has been validated for WB, ELISA, IHC, IF and IP. It is suitable to detect ISCU in samples from Human, Mouse and Rat.

Quick Overview for ISCU antibody (ABIN7115516)

Target: ISCU (Iron-sulfur cluster assembly enzyme ISCU, mitochondrial (ISCU))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ISCU antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-ISCU Antibody

Purpose: ISCU antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: iron-sulfur cluster scaffold homolog(E. coli)

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IP: 1:200-1:1000, IHC: 1:20-1:200, IF: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for ISCU

Target: ISCU (Iron-sulfur cluster assembly enzyme ISCU, mitochondrial (ISCU))

Alternative Name: ISCU

Background:

Synonyms: Iron-sulfur cluster assembly enzyme ISCU|NifU-like N-terminal domain-containing protein|NifU-like protein|ISCU|NIFUN

Background: Scaffold protein for the de novo synthesis of iron-sulfur(Fe-S) clusters within mitochondria, which is required for maturation of both mitochondrial and cytoplasmic [2Fe-2S] and [4Fe-4S] proteins(PubMed:11060020). First, a [2Fe-2S] cluster is transiently assembled on the scaffold protein ISCU. In a second step, the cluster is released from ISCU, transferred to a glutaredoxin GLRX5, followed by the formation of mitochondrial [2Fe-2S] proteins, the synthesis of [4Fe-4S] clusters and their target-specific insertion into the recipient apoproteins. Cluster assembly on ISCU depends on the function of the cysteine desulfurase complex NFS1-LYRM4/ISD11, which serves as the sulfur donor for cluster synthesis, the iron-binding protein frataxin as the putative iron donor, and the electron transfer chain comprised of ferredoxin reductase and ferredoxin, which receive their electrons from NADH(By similarity).

Molecular Weight: 14-15 kDa, 18-19 kDa

Gene ID: 23479

UniProt: Q9H1K1