POLR3A antibody
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- Target See all POLR3A Antibodies
- POLR3A (Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A))
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This POLR3A antibody is un-conjugated
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Application
- ELISA, Western Blotting (WB)
- Purification
- Immunogen affinity purified
- Purity
- ≥95 % as determined by SDS-PAGE
- Immunogen
- polymerase(RNA) III(DNA directed) polypeptide A, 155kDa
- Isotype
- IgG
- Top Product
- Discover our top product POLR3A Primary Antibody
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- Application Notes
- WB: 1:500-1:2000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- -20°C for 12 months (Avoid repeated freeze / thaw cycles.)
- Expiry Date
- 12 months
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- Target
- POLR3A (Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A))
- Alternative Name
- POLR3A (POLR3A Products)
- Background
- Synonyms: Background:DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition(By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs(EBERs) induce type I interferon and NF-Kappa-B through the RIG-I pathway.
- Molecular Weight
- 140-150 kDa
- Gene ID
- 11128
- UniProt
- O14802
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