RRP8 antibody

Catalog No. ABIN7118623

Product Details anti-RRP8 Antibody

Target: RRP8 (Ribosomal RNA Processing 8 (RRP8))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RRP8 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: ribosomal RNA processing 8, methyltransferase, homolog(yeast)

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IHC: 1:20-1:200, IF: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for RRP8

Target: RRP8 (Ribosomal RNA Processing 8 (RRP8))

Alternative Name: RRP8 (RRP8 Products)

Synonyms: KIAA0409 antibody, 1500003O22Rik antibody, 2900001K19Rik antibody, AW538116 antibody, RGD1308302 antibody, ribosomal RNA processing 8 antibody, ribosomal RNA processing 8, methyltransferase, homolog (yeast) antibody, RRP8 antibody, Rrp8 antibody

Background: Synonyms: KIAA0409, NML
Essential component of the eNoSC(energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9'(H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the complex, RRP8 binds to H3K9me2 and probably acts as a methyltransferase. Its substrates are however unknown.

Molecular Weight: 51 kDa

Gene ID: 23378

UniProt: O43159