RFWD3 antibody

Catalog No. ABIN7118631

This anti-RFWD3 antibody is a Rabbit Polyclonal antibody detecting RFWD3 in WB, ELISA, IHC and IF. Suitable for Human, Mouse and Rat.

Quick Overview for RFWD3 antibody (ABIN7118631)

Target: RFWD3 (Ring Finger and WD Repeat Domain 3 (RFWD3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RFWD3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-RFWD3 Antibody

Purpose: RFWD3 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: ring finger and WD repeat domain 3

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IHC: 1:20-1:200, IF: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for RFWD3

Target: RFWD3 (Ring Finger and WD Repeat Domain 3 (RFWD3))

Alternative Name: RFWD3

Background:

Synonyms: E3 ubiquitin-protein ligase RFWD3|RING finger and WD repeat domain-containing protein 3|RING finger protein 201|RFWD3|RNF201

Background: E3 ubiquitin-protein ligase that mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53. Plays a role in RPA-mediated DNA damage signaling and repair.

Molecular Weight: 85 kDa

Gene ID: 55159

UniProt: Q6PCD5