RNF34 antibody

Catalog No. ABIN7118654

The Rabbit Polyclonal anti-RNF34 antibody has been validated for ELISA and IHC. It is suitable to detect RNF34 in samples from Human, Mouse and Rat.

Quick Overview for RNF34 antibody (ABIN7118654)

Target: RNF34 (Ring Finger Protein 34 (RNF34))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF34 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Product Details anti-RNF34 Antibody

Purpose: RNF34 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: ring finger protein 34

Isotype: IgG

Application Details

Application Notes: IHC: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for RNF34

Target: RNF34 (Ring Finger Protein 34 (RNF34))

Alternative Name: RNF34

Background:

Synonyms: E3 ubiquitin-protein ligase RNF34|Caspase regulator CARP1|Caspases-8 and -10-associated RING finger protein 1 (CARP-1)|FYVE-RING finger protein Momo|Human RING finger homologous to inhibitor of apoptosis protein (hRFI)|RING finger protein 34|RING finger protein RIFF|RING-type E3 ubiquitin transferase RNF34|RNF34

Background: E3 ubiquitin-protein ligase that regulates several biological processes through the ubiquitin-mediated proteasomal degradation of various target proteins. Ubiquitinates the caspases CASP8 and CASP10, promoting their proteasomal degradation, to negatively regulate cell death downstream of death domain receptors in the extrinsic pathway of apoptosis(PubMed:15069192). May mediate 'Lys-48'-linked polyubiquitination of RIPK1 and its subsequent proteasomal degradation thereby indirectly regulating the tumor necrosis factor-mediated signaling pathway(Ref.13). Negatively regulates p53/TP53 through its direct ubiquitination and targeting to proteasomal degradation(PubMed:17121812). Indirectly, may also negatively regulate p53/TP53 through ubiquitination and degradation of SFN(PubMed:18382127). Mediates PPARGC1A proteasomal degradation probably through ubiquitination thereby indirectly regulating the metabolism of brown fat cells(PubMed:22064484). Possibly involved in innate immunity, through 'Lys-48'-linked polyubiquitination of NOD1 and its subsequent proteasomal degradation(PubMed:25012219).

Gene ID: 80196

UniProt: Q969K3