STT3A antibody

Catalog No. ABIN7119563

The Rabbit Polyclonal anti-STT3A antibody has been validated for ELISA, IHC, WB and IF. It is suitable to detect STT3A in samples from Human, Mouse and Rat.

Quick Overview for STT3A antibody (ABIN7119563)

Target: STT3A (STT3, Subunit of The Oligosaccharyltransferase Complex, Homolog A (STT3A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STT3A antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-STT3A Antibody

Purpose: STT3A antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: STT3, subunit of the oligosaccharyltransferase complex, homolog A

Isotype: IgG

Application Details

Application Notes: WB: 1:1000-1:5000, IHC: 1:20-1:200, IF: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for STT3A

Target: STT3A (STT3, Subunit of The Oligosaccharyltransferase Complex, Homolog A (STT3A))

Alternative Name: STT3A

Background:

Synonyms: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A (Oligosaccharyl transferase subunit STT3A, STT3-A)|B5|Integral membrane protein 1|Transmembrane protein TMC|STT3A|ITM1|TMC

Background: Catalytic subunit of the N-oligosaccharyl transferase(OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum(ER). SST3A seems to be involved in complex substrate specificity. STT3A is present in the majority of OST complexes and mediates cotranslational N-glycosylation of most sites on target proteins, while STT3B-containing complexes are required for efficient cotranslational glycosylation and mediate glycosylation of sites that have been skipped by STT3A.

Molecular Weight: 100 kDa

Gene ID: 3703

UniProt: P46977