Tubulin antibody (acLys40)

Catalog No. ABIN7120355

The Mouse Monoclonal anti-Tubulin antibody has been validated for WB, IF, ELISA and IHC. It is suitable to detect Tubulin in samples from Human, Mouse, Dog and Rat.

Quick Overview for Tubulin antibody (acLys40) (ABIN7120355)

Target: Tubulin (TUB)

Reactivity: Human, Mouse, Dog, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Tubulin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunohistochemistry (IHC)

Clone: 6A10

Product Details anti-Tubulin Antibody

Binding Specificity: acLys40

Purpose: Acetylated tubulin antibody

Purification: Protein A+G purification

Purity: ≥95 % as determined by SDS-PAGE

Immunogen:

Immunogen sequence: A synthetic peptide(1-50aa)

Immunogen: tubulin, alpha 1a

Isotype: IgG1

Application Details

Application Notes: WB: 1:500-1:5000, IF: 1:20-1:200, IHC: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for Tubulin

Target: Tubulin (TUB)

Alternative Name: tubulin

Background:

Synonyms: Tubulin alpha-1A chain|Alpha-tubulin 3|Tubulin B-alpha-1|Tubulin alpha-3 chain|Detyrosinated tubulin alpha-1A chain|TUBA1A|TUBA3

Background: Tubulin, composed of heterodimers of alpha and beta tubulin, is the mainly component of microtubules which play important roles in cell motility, mitosis, and intracellular vesicle transport. Both alpha and beta tubulin undergo several posttranslational modifications such as polyglutamylation and acetylation/deacetylation. Tubulin acetylation occurs on lysine-40 at the N-terminal of alpha tubulin and is conserved across species. The histone deacetylase HDAC6 and SIRT2 has been identified as tubulin deacetylases. Reversible acetylation of alpha tubulin may be implicated in regulating microtubule stability, cell motility, and axon regeneration. The level of acetylated tubulin has been linked to epithelial malignancies and sensitivity to chemotherapy. In addition, acetylated tubulin has been widely used as a marker for primary cilia. This antibody is specific to the acetylated tubulin, it does not recognize non-acetylated tubulin.

Molecular Weight: 52 kDa

Gene ID: 7846

UniProt: Q71U36