WT1 antibody

Catalog No. ABIN7120843

This Rabbit Polyclonal antibody specifically detects WT1 in IHC, IF, WB, ELISA and IP. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for WT1 antibody (ABIN7120843)

Target: WT1 (Wilms Tumor 1 (WT1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WT1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-WT1 Antibody

Purpose: WT1 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: Wilms tumor 1

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:5000, IHC: 1:50-1:500, IF: 1:20-1:200, IP: 1:500-1:5000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for WT1

Target: WT1 (Wilms Tumor 1 (WT1))

Alternative Name: WT1

Background:

Synonyms: Wilms tumor protein|WT33|WT1

Background: Transcription factor that plays an important role in cellular development and cell survival. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA.

Molecular Weight: 52 kDa

Gene ID: 7490

UniProt: P19544

Pathways: Tube Formation