WTAP antibody (AA 1-150)

Catalog No. ABIN7120845

This anti-WTAP antibody is a Mouse Monoclonal antibody detecting WTAP in WB, ELISA and IHC. Suitable for Human, Mouse and Rat.

Quick Overview for WTAP antibody (AA 1-150) (ABIN7120845)

Target: WTAP (Wilms Tumor 1 Associated Protein (WTAP))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This WTAP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone: 9D4

Product Details anti-WTAP Antibody

Binding Specificity: AA 1-150

Purpose: WTAP antibody

Purification: Protein A+G purification

Purity: ≥95 % as determined by SDS-PAGE

Immunogen:

Immunogen sequence: 1-150aa

Immunogen: Wilms tumor 1 associated protein

Isotype: IgG1

Application Details

Application Notes: WB: 1:1000-1:10000, IHC: 1:50-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for WTAP

Target: WTAP (Wilms Tumor 1 Associated Protein (WTAP))

Alternative Name: WTAP

Background:

Synonyms: Pre-mRNA-splicing regulator WTAP|Female-lethal(2)D homolog (hFL(2)D)|WT1-associated protein|Wilms tumor 1-associating protein|WTAP|KIAA0105

Background: Regulatory subunit of the WMM N6-methyltransferase complex, a multiprotein complex that mediates N6-methyladenosine(m6A) methylation of some adenosine residues of some mRNAs and plays a role in the efficiency of mRNA splicing, processing and mRNA stability. Required for accumulation of METTL3 and METTL14 to nuclear speckle(PubMed:24316715, PubMed:24407421, PubMed:24981863). Acts as a mRNA splicing regulator(PubMed:12444081). Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability(PubMed:17088532). Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes(PubMed:17095724).

Molecular Weight: 49 kDa

Gene ID: 9589

UniProt: Q15007