ZCCHC11 antibody

Catalog No. ABIN7121051

The Rabbit Polyclonal anti-ZCCHC11 antibody has been validated for WB, ELISA, IF and IP. It is suitable to detect ZCCHC11 in samples from Human and Mouse.

Quick Overview for ZCCHC11 antibody (ABIN7121051)

Target: ZCCHC11 (Zinc Finger, CCHC Domain Containing 11 (ZCCHC11))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZCCHC11 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-ZCCHC11 Antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: zinc finger, CCHC domain containing 11

Isotype: IgG

Application Details

Application Notes: WB: 1:200-1:2000, IP: 1:500-1:5000, IF: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for ZCCHC11

Target: ZCCHC11 (Zinc Finger, CCHC Domain Containing 11 (ZCCHC11))

Alternative Name: ZCCHC11

Background: Synonyms:KIAA0191, PAPD3, Terminal uridylyltransferase 4, TUT4, TUTase 4, ZCCHC11 Background:Uridylyltransferase that mediates the terminal uridylation of mRNAs with short(less than 25 nucleotides) poly(A) tails, hence facilitating global mRNA decay(PubMed:25480299). Involved in microRNA(miRNA)-induced gene silencing through uridylation of deadenylated miRNA targets. Also acts as a suppressor of miRNA biogenesis by mediating the terminal uridylation of some miRNA precursors, including that of let-7(pre-let-7), miR107, miR-143 and miR-200c. Uridylated miRNAs are not processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem(ES) cell pluripotency(By similarity). Does not bind RNA directly, but recruited to RNA targets by RNA-binding protein LIN28A. Also catalyzes the 3' uridylation of miR-26A, a miRNA that targets IL6 transcript. This abrogates the silencing of IL6 transcript, hence promoting cytokine expression(By similarity). May also suppress Toll-like receptor-induced NF-kappa-B activation via binding to T2BP. Does not play a role in replication-dependent histone mRNA degradation. Due to functional redundancy between ZCCHC6 and ZCCHC11, the identification of the specific role of each of these proteins is difficult.

Molecular Weight: 185 kDa

Gene ID: 23318

UniProt: Q5TAX3

Pathways: Stem Cell Maintenance