Tissue factor antibody (AA 32-100)

Catalog No. ABIN708086

The Rabbit Polyclonal anti-Tissue factor antibody has been validated for WB, ELISA, FACS, IHC (p), IF (cc), IF (p) and IHC (fro) and has been independently validated. It is suitable to detect Tissue factor in samples from Human, Mouse, Rat and Pig. There are 7+ publications available.

Quick Overview for Tissue factor antibody (AA 32-100) (ABIN708086)

Target: Tissue factor (F3) (Coagulation Factor III (thromboplastin, Tissue Factor) (F3))

Reactivity: Human, Mouse, Rat, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tissue factor antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Tissue factor Antibody

Binding Specificity: AA 32-100

Cross-Reactivity: Human, Mouse, Pig, Rat

Predicted Reactivity: Dog,Cow,Horse,Rabbit,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CD142

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Independent Validation (IHC)

Validation #029589 (Immunohistochemistry)

by: Reveal Biosciences

No.: #029589

Date: 02/07/2014

Lot Number: 980886W

Method validated: Immunohistochemistry

Positive Control: Mouse lung tissue

Negative Control: Mouse brain tissue

Notes: Coagulation Factor III (thromboplastin, Tissue Factor) (F3) staining is observed in positive control tissue and not in negative control tissue.

Validation Images

Figure 1: Mouse lung tissue stained with anti-F3 (brown) and counterstained in blue.

Figure 2: Mouse brain tissue stained with anti-F3 (brown) and counterstained in blue.

Figure 3: Mouse lung tissue stained with isotype control antibody (brown) and counterstained in blue.

Figure 4: Mouse lung tissue stained with secondary antibody only (brown) and counterstained in blue.

Full Methods

Primary Antibody:

• Antigen: Coagulation Factor III (thromboplastin, Tissue Factor) (F3)
• Catalog number: ABIN708086
• Lot number: 120224

Secondary Antibody:

• Antibody: Bond Polymer Refine Detection Kit
• Lot number: 24144

Full Protocol:

• Immunohistochemistry was performed on a Leica Bond automated immunostainer.
• Sections were deparaffinized with Novocastra Bond Dewax Solution and rehydrated into Leica Bond Wash Buffer.
• Sections were heated to 98 °C for 20 minutes in 10 mM citrate buffer pH 9.0 (ER1; Leica) for antigen retrieval.
• Sections were blocked in 3 % casein plus 0.1 % Triton-X100 for 10 minutes at room temperature.
• Sections were washed x 3 in Leica Bond Wash Buffer.
• Sections were incubated with primary antibody diluted 1:200 in Universal Antibody Dilution Buffer (Electron Microscopy Sciences, 25886-05) for 15 minutes at room temperature.
• Sections were washed x 3 in Leica Bond Wash Buffer.
• Sections were incubated with Leica Bond Polymer for 8 minutes at room temperature.
• Sections were washed x 4 in Leica Bond Wash Buffer.
• Sections were washed x 1 in Distilled Water.
• Sections were incubated with Peroxide Block (Leica) for 10 min to block endogenous peroxidase.
• Sections were washed x 4 in Leica Bond Wash Buffer.
• Sections were incubated with DAB chromogenic substrate (Leica) for 10 min at RT.
• Sections were washed x 3 in Distilled Water.
• Sections were counterstained with hematoxylin (Leica) for 2 minutes.
• Sections were washed x 1 in Distilled Water.
• Sections were washed x 1 in Leica Bond Wash Buffer.
• Sections were washed x 1 in Distilled Water.
• Sections were dehydrated, mounted and photographed under a light microscope.

Experimental Notes: None notes.

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Tissue factor antibody (ABIN708086)

Fletcher, Das, Gadye, Street, Baudhuin, Wagner, Cole, Flores, Choi, Yosef, Purdom, Dudoit, Risso, Ngai: "Deconstructing Olfactory Stem Cell Trajectories at Single-Cell Resolution." in: Cell stem cell, Vol. 20, Issue 6, pp. 817-830.e8, (2017) (PubMed).

Kim, Yoo, Gu, Kim: "Histones Induce the Procoagulant Phenotype of Endothelial Cells through Tissue Factor Up-Regulation and Thrombomodulin Down-Regulation." in: PLoS ONE, Vol. 11, Issue 6, pp. e0156763, (2016) (PubMed).

Yoo, Kim, Gu, Lee, Lee, Hwang, Hwang, Kim, Kim: "Porcine endothelium induces DNA-histone complex formation in human whole blood: a harmful effect of histone on coagulation and endothelial activation." in: Xenotransplantation, Vol. 23, Issue 6, pp. 464-471, (2016) (PubMed).

Gleeson, Martin, Ali, Kumar, Pillai, Kumar, OSullivan, Whelan, Stocca, Khider, Barry, OBrien, Caplice: "Bone Marrow-Derived Mesenchymal Stem Cells Have Innate Procoagulant Activity and Cause Microvascular Obstruction Following Intracoronary Delivery: Amelioration by Antithrombin Therapy." in: Stem cells (Dayton, Ohio), (2015) (PubMed).

Tian, Salsbery, Wang, Yuan, Yang, Zhao, Wu, Zhang, Konkle, Thiagarajan, Li, Zhang, Dong: "Brain-derived microparticles induce systemic coagulation in a murine model of traumatic brain injury." in: Blood, (2015) (PubMed).

Milano, Dongiovanni, Artoni, Gatti, Rosso, Colombo, Bollati, Maggioni, Mannucci, Bertazzi, Fargion, Valenti: "Particulate matter phagocytosis induces tissue factor in differentiating macrophages." in: Journal of applied toxicology : JAT, (2015) (PubMed).

Liu, Wei, Zhang, Xu, Wang, Zhou: "Procoagulant activity and cellular origin of microparticles in human amniotic fluid." in: Thrombosis research, Vol. 133, Issue 4, pp. 645-51, (2014) (PubMed).

Target Details for Tissue factor

Target: Tissue factor (F3) (Coagulation Factor III (thromboplastin, Tissue Factor) (F3))

Alternative Name: CD142

Background:

Synonyms: TF, TFA, CD142, Tissue factor, Coagulation factor III, Thromboplastin, F3

Background: Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.

Gene ID: 2152

UniProt: P13726

Pathways: Positive Regulation of Endopeptidase Activity, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling