CSTA antibody (AA 1-98)

Catalog No. ABIN7126264

This anti-CSTA antibody is a Mouse Monoclonal antibody detecting CSTA in WB, IHC and IHC (f). Suitable for Human.

Quick Overview for CSTA antibody (AA 1-98) (ABIN7126264)

Target: CSTA (Cystatin A (Stefin A) (CSTA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CSTA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-CSTA Antibody

Binding Specificity: AA 1-98

Specificity: The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins, and kininogens. This gene encodes a stefin that functions as a cysteine protease inhibitor, forming tight complexes with papain and the cathepsins B, H, and L. The protein is one of the precursor proteins of cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. Stefins have been proposed as prognostic and diagnostic tools for cancer.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment of human CSTA protein (around aa 1-98) (exact sequence is proprietary)

Isotype: IgG2b

Application Details

Application Notes:

Known_Application: Western Blot (2-4 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: THP-1 cells. Human tonsil or prostate tissue (IHC).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CSTA

Target: CSTA (Cystatin A (Stefin A) (CSTA))

Alternative Name: CSTA

Background: STF1, STFA, AREI, cstA, Stefin A,Cystatin A
Cellular localisation: Cell Surface, Cytoplasmic and Nuclear

Molecular Weight: 11kDa

Gene ID: 1475, 518198

UniProt: P01040

Pathways: Response to Water Deprivation