DAZL antibody (C-Term)

Catalog No. ABIN7126282

This anti-DAZL antibody is a Mouse Monoclonal antibody detecting DAZL in WB, IF and IHC (f). Suitable for Human.

Quick Overview for DAZL antibody (C-Term) (ABIN7126282)

Target: DAZL (Deleted in Azoospermia-Like (DAZL))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This DAZL antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-DAZL Antibody

Binding Specificity: C-Term

Specificity: Specific for human DAZL, a member of the DAZ (deleted in azoospermia) family of RNA binding proteins. DAZL is expressed in fetal and adult testes and ovaries, and is believed to play a role in germ cell development. In adult germ cells, the expression of DAZL is localized to the cytoplasm. Mutations in this gene have been linked to severe spermatogenic failure and infertility in males. Clone DAZL/4253 recognizes DAZL at its C terminus, and is used in detection of DAZL expression by various methods.

Cross-Reactivity (Details): Human, Mouse, Rat and Cynomolgus monkey.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recognizes DAZL protein C terminus sequence CRVHHFRRSRAMLKSV.

Isotype: IgG1

Application Details

Application Notes:

Known_Application: Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human testis or ovary tissue.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for DAZL

Target: DAZL (Deleted in Azoospermia-Like (DAZL))

Alternative Name: DAZL

Background: Deleted in azoospermia-like, DAZL, DAZ homolog, DAZ-like autosomal, Deleted in azoospermia-like 1, SPGY-like-autosomal, DAZH, DAZL1, DAZLA, SPGYLA,DAZL (Deleted in Azoospermia-like)
Cellular localisation: Cytoplasmic

Molecular Weight: 33kDa

Gene ID: 1618, 131179

UniProt: Q92904