EIF2S1 antibody

Catalog No. ABIN7126320

Product Details anti-EIF2S1 Antibody

Target: EIF2S1 (Eukaryotic Translation Initiation Factor 2 Subunit 1 (EIF2S1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EIF2S1 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Specificity: The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex is composed of three subunits, designated eIF2a, eIF2band eIF2g (eukaryotic translation initiation factor 2 a, band g, respectively), all of which work in concert to form a ternary complex with GTP and tRNA in the early stages of protein synthesis. eIF2a, also known as EIF2S1 or EIF2, is a 315 amino acid subunit of the eukaryotic initiation complex that functions to bind tRNA to the 40S ribosomal subunit (in a GTP-dependent manner), thereby initiating translation. In addition, the phosphorylation state of eIF2a controls the rate of tRNA translation. When eIF2a is not phosphorylated, translation occurs at a normal rate. However, upon phosphorylation by one of several kinases, eIF2a is stabilized, thus preventing the GDP/GTP exchange reaction and slowing translation.

Cross-Reactivity (Details): Human. Predicted to work in Rat and Zebrafish.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human EIF2S1 protein

Isotype: IgG2a

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or MCF7 cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for EIF2S1

Target: EIF2S1 (Eukaryotic Translation Initiation Factor 2 Subunit 1 (EIF2S1))

Alternative Name: EIF2S1 (EIF2S1 Products)

Background: Eukaryotic translation initiation factor 2 subunit 1 alpha 35 kDa (EIF2S1), EIF-2alpha, EIF2, EIF2A,EIF2S1 / EIF-2 alpha (EIF2A)
Cellular localisation: Nucleus.

Molecular Weight: 36kDa

Gene ID: 1965, 151777

UniProt: P05198

Pathways: Ribonucleoprotein Complex Subunit Organization, ER-Nucleus Signaling, Hepatitis C