EIF4E antibody

Catalog No. ABIN7126325

This Mouse Monoclonal antibody specifically detects EIF4E in WB, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for EIF4E antibody (ABIN7126325)

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EIF4E antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-EIF4E Antibody

Specificity: The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex eIF4F exists in vitro as a trimeric complex of eIF4E, eIF4A and eIF4G. Together, the complex allows ribosome binding to mRNA by inducing the unwinding of mRNA secondary structures. eIF4E binds to the mRNA cap during an early step in the initiation of protein synthesis. eIF4A acts as an ATP-dependent RNA helicase. eIF4G acts as a bridge between eIF4E, eIF4A and the eIF3 complex.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human EIF4E protein

Isotype: IgG2a

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), ,Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa, Raji or U87 cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for EIF4E

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Alternative Name: EIF4E

Background: AUTS19, CBP, eIF-4F 25 kDa subunit, eIF4E, EIF4E1, EIF4EL1, EIF4F, Eukaryotic translation initiation factor 4E like 1, Messanger RNA (mRNA) Cap Binding Protein eIF 4E,EIF4E
Cellular localisation: Cytoplasm.

Molecular Weight: 25.1kDa

Gene ID: 1977, 249718

UniProt: P06730

Pathways: BCR Signaling