Recombinant KRT17 antibody

Catalog No. ABIN7126634

This Rabbit Monoclonal antibody specifically detects KRT17 in IHC (f). It exhibits reactivity toward Human.

Quick Overview for Recombinant KRT17 antibody (ABIN7126634)

Target: KRT17 (Keratin 17 (KRT17))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This KRT17 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-KRT17 Antibody

Specificity: Cytokeratin 17 (CK17) is normally expressed in the basal cells ofcomplex epithelia but not in stratified orsimple epithelia. Antibody to CK17 is an excellent tool to distinguish myoepithelial cells from luminal epithelium of various glandssuch as mammary, sweat and salivary. CK17 is expressed in epithelial cells of various origins, such as bronchial epithelial cells and skin appendages. It may be considered as epithelial stem cell marker because CK17 Ab marks basal cell differentiation. CK17 is expressed in SCLC much higher than in LADC. Eighty-five percent of the triple negative breast carcinomas immunoreact with basal cytokeratins including anti-CK17. Also important is that cases of triple negative breast carcinoma with expression of CK17 show an aggressive clinical course. Additionally, anti-CK17 and anti-MUC1 immunoreactivity represents pancreatobiliary subtype whereas anti-MUC2 and anti-CDX-2 positivity defines intestinal subtype.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human KRT17 protein

Isotype: IgG

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: T24 cells. Humanskin, bladder or cervix.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for KRT17

Target: KRT17 (Keratin 17 (KRT17))

Alternative Name: KRT17

Background: K17, Keratin Type I Cytoskeletal 17, Keratin-17, KRT17, PCHC1,Cytokeratin 17 (CK17) (Basal Epithelial Marker)
Cellular localisation: Cytoplasm.

Molecular Weight: 46kDa

Gene ID: 3872, 2785

UniProt: Q04695