Lactoferrin antibody (AA 614-645)

Catalog No. ABIN7126646

This Mouse Monoclonal antibody specifically detects Lactoferrin in IF, FACS and IHC (f). It exhibits reactivity toward Human.

Quick Overview for Lactoferrin antibody (AA 614-645) (ABIN7126646)

Target: Lactoferrin (LTF) (Lactotransferrin (LTF))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Lactoferrin antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-Lactoferrin Antibody

Binding Specificity: AA 614-645

Specificity: Ferritin and transferrins manage necessary iron-binding functions for iron metabolism. Transferrins comprise a class of single-chain, two-sited, metal-binding proteins expressed throughout the fluid and cells of vertebrates. The three major types of transferrin include serotransferrin, lactotransferrin (lactoferrin) and ovotransferrin. Lactoferrin is found in milk, tears and leukocytes. It degrades an IgA1protease secreted by Haemophilus influenzae and, consequently, allows the human IgA1antibody to effectively abolish Haemophilus influenzae colonization. Lactoferrin also attenuates the pathogenic potential of Haemophilus influenzae by proteolytic degradation of the Hap adhesin. While lactoferrin may aid in the transmission of human T cell leukemiavirus type 1, it inhibits HIV-1 replication at the level of viral fusion and entry into cells. The inhibitory effects of lactoferrin on mixed lymphocyte reactions suggest that it may have the ability to sense the activation status of lymphocytes.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa614-645) of human Lactotransferrin protein (exact sequence is proprietary)

Isotype: IgG2a

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7 cells. Human lactating breast or prostate tissue.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Lactoferrin

Target: Lactoferrin (LTF) (Lactotransferrin (LTF))

Alternative Name: LTF

Background: GIG12, Growth inhibiting protein 12, HLF2, Kaliocin 1, Lactoferrin, Lactoferroxin-C, Lactotransferrin, LF, LTF, Neutrophil lactoferrin, Talalactoferrin,Lactotransferrin / Lactoferrin / LTF
Cellular localisation: Cytoplasm. Secreted.

Molecular Weight: 78kDa

Gene ID: 4057, 529517

Pathways: Transition Metal Ion Homeostasis