MBP antibody

Catalog No. ABIN7126676

Product Details anti-MBP Antibody

Target: MBP

Reactivity: Rat, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This MBP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunocytochemistry (ICC), Coating (Coat)

Specificity: Myelin basic protein (MBP) is the second most abundant protein in central nervous system (CNS) myelin: it comprises 30 % of the total protein and about 10 % of the dry weight of myelin. It is the only structural protein found so far to be essential for formation of CNS myelin, and has been called the executive molecule of myelin . MBP can interact with a number of polyanionic proteins including actin, tubulin, calmodulin, and clathrin, and negatively charged lipids, and acquires structure on binding to them. It may act as a membrane actin-binding protein, which might allow it to participate in transmission of extracellular signals to the cytoskeleton in oligodendrocytes and tight junctions in myelin. MBP may be applicable as a marker for oligodendrogliomas. MBP/4275 is used in clinical diagnostics to detect MBP levels or myelination in human MBP. MBP/4275 is used in a two-site ELISA with clone MBP/4273. Both are used interchangeably as capture and detection antibody.

Cross-Reactivity (Details): Human, Chicken, Guinea Pig, Rat, Pig, Rabbit, Sheep and Mammals.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Spleen cells from an immunised outbred rat were fused with cells of the mouse NS0 myeloma cell line.

Isotype: IgG2a

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA), Immunocytochemistry (0.5-1 μg/mL), ,Western Blot (1-2 μg/mL), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human brain or astrocytoma tissue.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for MBP

Target: MBP

Abstract: MBP Products

Background: GDB, Golli MBP, myelin basic protein, Hemopoietic MBP, HMBPR, HUGO, MLD, Myelin A1 Protein, basic, Myelin Deficient, Myelin membrane encephalitogenic protein, SHI, Shiverer, SP,Myelin Basic Protein
Cellular localisation: Myelin membrane, Peripheral membrane protein, Cytoplasmic side (of myelin).

Molecular Weight: 13-21kDa

Gene ID: 4155, 551713

UniProt: P02686