MEF2D antibody

Catalog No. ABIN7126680

The Mouse Monoclonal anti-MEF2D antibody has been validated for FACS, IP, IF and IHC (f). It is suitable to detect MEF2D in samples from Human.

Quick Overview for MEF2D antibody (ABIN7126680)

Target: MEF2D (Myocyte Enhancer Factor 2D (MEF2D))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MEF2D antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-MEF2D Antibody

Specificity: The myocyte enhancer factor-2 (MEF-2) family of transcription factors associate with co-repessors or co-activators to regulate development and function of T cells, neuronal cells and muscle cells. Four family members arise from alternatively spliced transcripts, termed MEF-2A, -2B, -2C and -2D. These members bind as homo- and heterodimers to the MEF-2 site in the promoter region of affected genes. Differential regulation in the expression of the four transcripts implies functional distinction for each duing embryogenesis and development. The process of differentiation from mesodermal precursor cells to myoblasts has led to the discovery of a variety of tissue-specific factors that regulate muscle gene expression. The myogenic basic helix-loop-helix proteins, including MyoD, myogenin, Myf-5 and MRF4, are one class of identified factors. A second family of DNA-binding regulatory proteins is the myocyte-specific enhancer factor-2 (MEF-2) family. Each of these proteins binds to the MEF-2 target DNA sequence present in the regulatory regions of many muscle-specific genes.

Cross-Reactivity (Details): Human. Predicted to react in Mouse and Rat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human MEF2D protein

Isotype: IgG2b

Application Details

Application Notes:

Known_Application: Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for MEF2D

Target: MEF2D (Myocyte Enhancer Factor 2D (MEF2D))

Alternative Name: MEF2D

Background: MADS box transcription factor 2 polypeptide D, Myocyte specific enhancer factor 2, polypeptide D,MEF2D / Myocyte enhancer factor 2D (Transcription Factor)
Cellular localisation: Nucleus. Translocated by HDAC4 to nuclear dots.

Molecular Weight: 55.94kDa

Gene ID: 4209, 314327

UniProt: Q14814