Recombinant Myeloperoxidase antibody (AA 150-250)

Catalog No. ABIN7126714

This anti-Myeloperoxidase antibody is a Rabbit Monoclonal antibody detecting Myeloperoxidase in WB, IHC (f) and BP. Suitable for Human.

Quick Overview for Recombinant Myeloperoxidase antibody (AA 150-250) (ABIN7126714)

Target: Myeloperoxidase (MPO)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Myeloperoxidase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Blocking Peptide (BP)

Product Details anti-Myeloperoxidase Antibody

Binding Specificity: AA 150-250

Specificity: Myeloperoxidase (MPO) also called the peroxidase (POD), is an important marker of bone marrow cells. It is one of the members of the family of heme peroxidase super existing in myeloid cells (mainly neutrophils and monocytes of aniline blue particles). With the deepening of the research on MPO, MPO gene polymorphism has been found to lead to individual for some disease susceptibility differences, with a variety of human development is closely related to the occurrence of diseases. The antibody reacts with neutrophil granulocytes and monocytes in blood and with precursors of granulocytes in the bone marrow. The antibody is useful as an aid for classification of neoplastic tissue, i.e. myeloblasts and immature myeloid cells of acute myelogenous leukemia, progranulocytic leukemia, monomyelocytic leukemia, erythroleukemia and myeloblastoma.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa150-250) of human MPO protein (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human B cell lymphoma or liver tissue. Spleen tissue lysate.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Myeloperoxidase

Target: Myeloperoxidase (MPO)

Alternative Name: MPO

Background: 84 kDa / 89 kDa myeloperoxidase, EC 1.11.1.7, EC1.11.2.2, fj80f04, MPO, mpx, myeloid-specific peroxidase, Myeloperoxidase, Myeloperoxidase heavy chain, Myeloperoxidase light chain,Myeloperoxidase / MPO
Cellular localisation: Cytoplasm.

Molecular Weight: heavy-light promoter: 72kDa, dimer: 140kDa

Gene ID: 4353, 458272

UniProt: P05164

Pathways: Chromatin Binding