Myeloperoxidase antibody (AA 150-250)

Catalog No. ABIN7126715

This Rabbit Polyclonal antibody specifically detects Myeloperoxidase in WB, IHC (f) and BP. It exhibits reactivity toward Human.

Quick Overview for Myeloperoxidase antibody (AA 150-250) (ABIN7126715)

Target: Myeloperoxidase (MPO)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Myeloperoxidase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Blocking Peptide (BP)

Product Details anti-Myeloperoxidase Antibody

Binding Specificity: AA 150-250

Specificity: The heme protein myeloperoxidase (MPO) is a major component of azurophilicgranules of neutrophils and polymorphonuclear leukocytes. Optimal oxygen-dependent microbiocidal activity depends on MPO as the critical enzyme forthe generation of hypochlorous acid and other toxic oxygen products. TheMPO precursor is synthesized during the promyelocytic stage of myeloid dif-ferentiation and is subsequently processed and transported intracellularly tothe lysosomes. The precursor undergoes cotranslational N-linked glycosyla-tion to produce a glycoprotein. Glucosidases in the endoplasmic reticulum(ER) or early cisGolgi convert the pro-MPO to a form which is sorted into aprelysosomal compartment, which undergoes final proteolytic maturation tonative MPO, a pair of heavy-light protomers. In normal neutrophils, MPO isexpressed as a dimer. Calreticulin, a calcium-binding protein residing in theER, interacts specifically with fully glycosylated apopro-MPO. iMPO mRNA isabundant in human promyelocytic HL-60 and mouse myeloid leukemia NFS-60cells. MPO is expressed at high levels in circulating neutrophils and mono-cytes but is not detectable in microglia, brain-specific macrophages or normalbrain tissue.MPO, which has a molecular weight of approximately 140 kD, is homodimer that can be split into two halves that still have enzymatic activity. These hemi-MPOmonomersconsist of a 59-kD alpha chainand a 13.5-kD beta chain.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Synthetic peptide corresponding to residues within aa150-250 of Myeloperoxidase was used as an immunogen.

Isotype: IgG

Application Details

Application Notes:

Known_Application: Western Blot (2-4 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human B cell lymphoma or spleen or liver tissue.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Myeloperoxidase

Target: Myeloperoxidase (MPO)

Alternative Name: MPO

Background: 84 kDa / 89 kDa myeloperoxidase, EC 1.11.1.7, EC1.11.2.2, fj80f04, MPO, mpx, myeloid-specific peroxidase, Myeloperoxidase, Myeloperoxidase heavy chain, Myeloperoxidase light chain,Myeloperoxidase / MPO
Cellular localisation: Cytoplasmic.

Molecular Weight: heavy-light promoter: 72kDa, dimer: 140kDa

Gene ID: 4353, 458272

UniProt: P05164

Pathways: Chromatin Binding