Neurogenin 3 antibody

Catalog No. ABIN7126754

This Mouse Monoclonal antibody specifically detects Neurogenin 3 in ELISA, IP, FACS and Coat. It exhibits reactivity toward Human.

Quick Overview for Neurogenin 3 antibody (ABIN7126754)

Target: Neurogenin 3 (NEUROG3)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Neurogenin 3 antibody is un-conjugated

Application: ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS), Coating (Coat)

Product Details anti-Neurogenin 3 Antibody

Specificity: This antigen is a transcription factor that interacts with NKX2-2 to regulation transcription of NEUROD1.The neurogenin family of proteins belongs to the basic helix-loop-helix (bHLH) superfamily and consists of Neurogenin 1, Neurogenin 2 and Neurogenin 3 (also designated ngn3). bHLH members are transcriptional regulators that determine cell fate. Neurogenin 3 is expressed in discrete regions of developing neurons and in the embryonic pancreatic islets of Langerhans. HNF-6 (hepatocyte nuclear factor 6) acts as a positive regulator of Neurogenin 3 by binding to and stimulating the neurogenin gene promoter. Neurogenin 3 is involved in the initial differentiation of the four islets cell types, while a network of transcription factors, including Hlxb9, Isl1, NeuroD, Nkx-2.2, Nkx-6.4, Pax-4, Pax-6, PDX-1 and Mash1, are required for final differentiation. Neurogenin 3 acts upstream of NeuroD in a bHLH cascade. Neurogenin 3 activates the expression of NeuroD at the onset of islet cell differentiation through box sequences E1 and E3 in the NeuroD promoter.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human full-lengthNEUROG3protein

Isotype: IgG2b

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA), ,Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells. Human fetal gut, colon, liver or heart.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Neurogenin 3

Target: Neurogenin 3 (NEUROG3)

Alternative Name: NEUROG3

Background: ATH4B, Atoh5, bHLHa7, Class A basic helix-loop-helix protein 7, Math4B, NEUROG3, Neurogenin-3, Neurogenin3, NGN-3, ngn3, Protein atonal homolog 5,NEUROG3 / Neurogenin 3 / NGN3 (Transcription Factor)
Cellular localisation: Nucleus.

Molecular Weight: 23.54kDa

Gene ID: 50674, 288655

UniProt: Q9Y4Z2