TIM3 antibody (AA 22-202)
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- Target See all TIM3 (TIM 3) Antibodies
- TIM3 (TIM 3) (Hepatitis A Virus Cellular Receptor 2 (TIM 3))
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Binding Specificity
- AA 22-202
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This TIM3 antibody is un-conjugated
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Application
- Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificity
- TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk. TIM-3 is expressed on the surface of effector T cel tonsil, lymph node or spleen. ls (CD4+Th1 and CD8+Tc1) but not on helper T cells (CD4+Th2 and CD8+Tc2). In chronic inflammation, autoimmune disorders, and some cancers, TIM-3 is upregulated on several other hematopoietic cell types. The Ig domain of TIM-3 interacts with a ligand on resting but not activated Th1 and Th2 cells. The glycosylated Ig domain of TIM-3 binds cell-associated galectin-9. This induces TIM-3 Tyr phosphorylation and pro-apoptotic signaling. TIM-3 functions as a negative regulator of Th1 cell activity. Its blockade results in increased IFN-gamma production, Th1 cell proliferation and cytotoxicity, regulatory T cell development, and increases in macrophage and neutrophil infiltration into sites of inflammation.
- Cross-Reactivity (Details)
- Human.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Recombinant fragment of human TIM3 protein (around aa 22-202) (exact sequence is proprietary)
- Isotype
- IgG2b
- Top Product
- Discover our top product TIM 3 Primary Antibody
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- Application Notes
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Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: HepG2 or Daudi cells. Human tonsil, lymph node or spleen.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
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- Target
- TIM3 (TIM 3) (Hepatitis A Virus Cellular Receptor 2 (TIM 3))
- Alternative Name
- HAVCR2 (TIM 3 Products)
- Synonyms
- HAVCR2 antibody, MGC140131 antibody, HAVcr-2 antibody, KIM-3 antibody, TIM3 antibody, TIMD-3 antibody, TIMD3 antibody, Tim-3 antibody, TIM-3 antibody, Tim3 antibody, Timd3 antibody, tim3 antibody, hepatitis A virus cellular receptor 2 antibody, HAVCR2 antibody, Havcr2 antibody
- Target Type
- Virus
- Background
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CD366, HAVR2, Hepatitis A virus cellular receptor 2 (HAVCR2), Kidney injury molecule 3 (KIM3), T-cell immunoglobulin and mucin domain-containing protein 3, T-cell immunoglobulin mucin receptor 3, T-cell membrane protein 3, TIM3, TIMD3,TIM3 / HAVCR2 / CD366 (Effector T-Cell Marker)
Cellular localisation: Cell Surface - Molecular Weight
- 60kDa
- Gene ID
- 84868, 710500
- UniProt
- Q8TDQ0
- Pathways
- Regulation of Lipid Metabolism by PPARalpha, Cancer Immune Checkpoints
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